Quantitative microscopy of human DNA topoisomerase II-α expression in transitional cell carcinoma of the bladder

Lea Rath-Wolfson, Ilan Hammel, Valentina Kugel, Zvi Leib, Yoram Dekel, Rumelia Koren

Research output: Contribution to journalArticlepeer-review

Abstract

OBJECTIVE: To compare the nuclear size of various grades of transitional cell carcinoma (TCC) stained immunohistochemically with the nuclear enzyme topoisomerase II-α (topo II-α) in bladder urothelial neoplasms. STUDY DESIGN: Histologic sections from 53 consecutive papillary bladder neoplasms were stained immunohistochemically for topo II-α expression. There were 18 (33.9%) urothelial neoplasms of low malignant potential (UNLMP), 18 (33.9%) low grade urothelial carcinoma (LGUCa), and 17 (32%) with high grade urothelial carcinoma (HGUCa). The histologic slides were photographed at 400 × magnification and then projected on a screen, and the area with stained nuclei was measured. RESULTS: The cells and nuclei in HGUCa were significantly larger than in LGUCa (P < .05) and UNLMP (P < .01). CONCLUSION: Calculation of the area fraction of nuclei in TCC of the bladder stained with topo II-α is an additional method of establishing the grade of these tumors.

Original languageEnglish
Pages (from-to)181-186
Number of pages6
JournalAnalytical and Quantitative Cytology and Histology
Volume26
Issue number4
StatePublished - Aug 2004

Keywords

  • Bladder cancer
  • Carcinoma
  • DNA topoisomerase
  • Morphometry
  • Transitional cell

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