Quantitative measurements of mouse brain thrombin-like and thrombin inhibition activities

Orit Beilin, David Gurwitz, Amos D. Korczyn, Joab Chapman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Thrombin-like enzymatic activity was measured in mouse brain homogenates and slices by cleavage of a peptide substrate, N-p-Tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin. The activity was localized mainly to white matter. However, it was not affected by specific thrombin inhibitors, and was found to represent the sum of at least two enzyme activities, a prolyl endopeptidase and an aminopeptidase. By specifically inhibiting this endogenous activity in combination with exogenously added thrombin, mouse brain tissue was shown to express a capacity of thrombin inhibitory activity equivalent to 0.2 mU thrombin/mg brain tissue. The present study offers a simple and reliable method for measuring total thrombin inhibitory activity in brain.

Original languageEnglish
Pages (from-to)2347-2351
Number of pages5
Issue number11
StatePublished - 8 Aug 2001


  • Bestatin
  • Coagulation
  • Hirudin
  • Prolyl endopeptidase
  • Protease nexin-I
  • Prothrombin
  • Serpins
  • Thrombin


Dive into the research topics of 'Quantitative measurements of mouse brain thrombin-like and thrombin inhibition activities'. Together they form a unique fingerprint.

Cite this