Quantitative measurements obtained by micro-computed tomography and confocal laser scanning microscopy

Kivanç Kamburoǧlu, S. F. Barenboim, T. Antürk, Israel Kaffe

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: To compare measurements obtained by micro-CT with those obtained by confocal laser scanning microscope in simulative internal resorption cavities. Methods: An extracted human maxillary central incisor tooth was divided into two in the coronal plane. Four artificial internal resorption cavities were prepared with standardized burs on each section, and diameters and volumes were measured using a laser scanning electron confocal microscope and a desktop cone beam micro-CT-40. Differences between means of quantitative measurements for both methods were tested using the paired t-test; the correlation between quantitative measurements was tested by regression analysis. Results: Mean diameter and volume differences between the two methods were 0.061 mm and 0.004 mm3, respectively. Average diameter and volume calculations obtained by micro-CT were significantly lower (P < 0.001 for diameter and P < 0.01 for volume). A significantly strong relationship was found in the average diameters (R2 = 92.9%) and calculated volumes (R2 = 91%), (P < 0.001). Conclusions: Although a strong correlation between both methods was found, micro-CT significantly underestimated the diameters and volumes.

Original languageEnglish
Pages (from-to)385-391
Number of pages7
JournalDentomaxillofacial Radiology
Volume37
Issue number7
DOIs
StatePublished - Oct 2008

Keywords

  • Internal resorption
  • Micro-computed tomography
  • Quantitative measurement
  • Scanning electron confocal microscopy

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