TY - JOUR
T1 - Quantitative mass spectrometry analysis reveals a panel of nine proteins as diagnostic markers for colon adenocarcinomas
AU - Atak, Apurva
AU - Khurana, Samiksha
AU - Gollapalli, Kishore
AU - Reddy, Panga Jaipal
AU - Levy, Roei
AU - Ben-Salmon, Stav
AU - Hollander, Dror
AU - Donyo, Maya
AU - Heit, Anke
AU - Hotz-Wagenblatt, Agnes
AU - Biran, Hadas
AU - Sharan, Roded
AU - Rane, Shailendra
AU - Shelar, Ashutosh
AU - Ast, Gil
AU - Srivastava, Sanjeeva
N1 - Publisher Copyright:
© Atak et al.
PY - 2018
Y1 - 2018
N2 - Adenocarcinomas are cancers originating from the gland forming cells of the colon and rectal lining, and are known to be the most common type of colorectal cancers. The current diagnosis strategies for colorectal cancers include biopsy, laboratory tests, and colonoscopy which are time consuming. Identification of protein biomarkers could aid in the detection of colon adenocarcinomas (CACs). In this study, tissue proteome of colon adenocarcinomas (n = 11) was compared with the matched control specimens (n = 11) using isobaric tags for relative and absolute quantitation (iTRAQ) based liquid chromatography-mass spectrometry (LC-MS/MS) approach. A list of 285 significantly altered proteins was identified in colon adenocarcinomas as compared to its matched controls, which are associated with growth and malignancy of the tumors. Protein interaction analysis revealed the association of altered proteins in colon adenocarcinomas with various transcription factors and their targets. A panel of nine proteins was validated using multiple reaction monitoring (MRM). Additionally, S100A9 was also validated using immunoblotting. The identified panel of proteins may serve as potential biomarkers and thereby aid in the detection of colon adenocarcinomas.
AB - Adenocarcinomas are cancers originating from the gland forming cells of the colon and rectal lining, and are known to be the most common type of colorectal cancers. The current diagnosis strategies for colorectal cancers include biopsy, laboratory tests, and colonoscopy which are time consuming. Identification of protein biomarkers could aid in the detection of colon adenocarcinomas (CACs). In this study, tissue proteome of colon adenocarcinomas (n = 11) was compared with the matched control specimens (n = 11) using isobaric tags for relative and absolute quantitation (iTRAQ) based liquid chromatography-mass spectrometry (LC-MS/MS) approach. A list of 285 significantly altered proteins was identified in colon adenocarcinomas as compared to its matched controls, which are associated with growth and malignancy of the tumors. Protein interaction analysis revealed the association of altered proteins in colon adenocarcinomas with various transcription factors and their targets. A panel of nine proteins was validated using multiple reaction monitoring (MRM). Additionally, S100A9 was also validated using immunoblotting. The identified panel of proteins may serve as potential biomarkers and thereby aid in the detection of colon adenocarcinomas.
KW - Biomarkers
KW - Colon adenocarcinoma
KW - ITRAQ
KW - Proteomics
KW - Tissue
UR - http://www.scopus.com/inward/record.url?scp=85042720337&partnerID=8YFLogxK
U2 - 10.18632/oncotarget.24418
DO - 10.18632/oncotarget.24418
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C2 - 29568375
AN - SCOPUS:85042720337
SN - 1949-2553
VL - 9
SP - 13530
EP - 13544
JO - Oncotarget
JF - Oncotarget
IS - 17
ER -
