Quantitative mass spectrometry analysis reveals a panel of nine proteins as diagnostic markers for colon adenocarcinomas

Apurva Atak, Samiksha Khurana, Kishore Gollapalli, Panga Jaipal Reddy, Roei Levy, Stav Ben-Salmon, Dror Hollander, Maya Donyo, Anke Heit, Agnes Hotz-Wagenblatt, Hadas Biran, Roded Sharan, Shailendra Rane, Ashutosh Shelar, Gil Ast, Sanjeeva Srivastava*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Adenocarcinomas are cancers originating from the gland forming cells of the colon and rectal lining, and are known to be the most common type of colorectal cancers. The current diagnosis strategies for colorectal cancers include biopsy, laboratory tests, and colonoscopy which are time consuming. Identification of protein biomarkers could aid in the detection of colon adenocarcinomas (CACs). In this study, tissue proteome of colon adenocarcinomas (n = 11) was compared with the matched control specimens (n = 11) using isobaric tags for relative and absolute quantitation (iTRAQ) based liquid chromatography-mass spectrometry (LC-MS/MS) approach. A list of 285 significantly altered proteins was identified in colon adenocarcinomas as compared to its matched controls, which are associated with growth and malignancy of the tumors. Protein interaction analysis revealed the association of altered proteins in colon adenocarcinomas with various transcription factors and their targets. A panel of nine proteins was validated using multiple reaction monitoring (MRM). Additionally, S100A9 was also validated using immunoblotting. The identified panel of proteins may serve as potential biomarkers and thereby aid in the detection of colon adenocarcinomas.

Original languageEnglish
Pages (from-to)13530-13544
Number of pages15
JournalOncotarget
Volume9
Issue number17
DOIs
StatePublished - 2018

Funding

FundersFunder number
University Grants CommissionF.6-17/2014

    Keywords

    • Biomarkers
    • Colon adenocarcinoma
    • ITRAQ
    • Proteomics
    • Tissue

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