TY - JOUR
T1 - Quantitative enzymatic assay of short-chain acylcarnitines after separation by paper chromatography
AU - Golan, R.
AU - Lewin, L. M.
N1 - Funding Information:
This research has been supported in part, by a grant from the Office of the Chief Scientist, Ministry of Health, State of Israel, to Professor L. M. Lewin.
PY - 1980/6
Y1 - 1980/6
N2 - Esters of carnitine with fatty acids containing two to five carbon atoms were separated by descending chromatography on Whatman No. 1 paper, using the solvent system, n-butanol: acetic acid:water (8:1:1, v/v). The separated derivatives were hydrolyzed with ammonia; the resulting l-carnitine was eluted from each chromatographic zone and assayed enzymatically. This procedure permits separation and assay of nanomole amounts of acylcarnitines from biological materials.
AB - Esters of carnitine with fatty acids containing two to five carbon atoms were separated by descending chromatography on Whatman No. 1 paper, using the solvent system, n-butanol: acetic acid:water (8:1:1, v/v). The separated derivatives were hydrolyzed with ammonia; the resulting l-carnitine was eluted from each chromatographic zone and assayed enzymatically. This procedure permits separation and assay of nanomole amounts of acylcarnitines from biological materials.
UR - http://www.scopus.com/inward/record.url?scp=0019195701&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(80)90455-8
DO - 10.1016/0003-2697(80)90455-8
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AN - SCOPUS:0019195701
SN - 0003-2697
VL - 105
SP - 264
EP - 267
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -