An in vitro bioassay for the quantitative detection of erythropoietin (Ep) in human plasma is described. The method is based on the increased 3H-uridine incorporation into 12-day embryonic mouse liver erythroblasts due to Ep. It was found that 11 out of 12 anaemic patients showed high plasma Ep level, while 7 out of 8 patients suffering from polycythaemia revealed low Ep values. In 4 out of 8 patients with chronic renal failure the Ep level was within the normal range, whereas in the remaining 4 it was higher than normal. The relative simplicity of the method and the reproducibility of the results make it useful even in the routine laboratory.
- Human plasma erythropoietin
- Mouse liver