Quantitative comparison of cytokine mRNA and inflammatory responses in cutaneous late phase allergic reactions

Burton Zweiman*, Carmen M. Parrott, Yael Graif, Mary David, Stuart R. Lessin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The aim of this study was to quantitatively compare expression of mRNA for IL-5 and IFN-γ with the frequency of mRNA-positive cells, total and activated eosinophils, neutrophils, lymphocytes, and vessels expressing adhesion molecules. Replicate biopsies of skin LPR to pollen antigens (Ag) and control injection sites (B) at 6 and 24 h were assessed for: (1) mRNA for IL-5 and IFN-γ by quantitative RT-PCR (QC-RT/PCR); (2) frequency of cells expressing mRNA for IL-5 and IFN-γ by in situ hybridization (ISH); (3) inflammatory cells and adhesion molecule expression. More mRNA for IL-5 was found in Ag- than in B-injected sites at 6 and 24 h by both QC-RT/PCR and ISH. Small amounts of mRNA for IFN-γ were detected in Ag sites by QC-RT/PCR at 6 and 24 h, but were not significantly different than at B sites. The frequency of IFN-γ mRNA+ cells was higher in Ag than in B sites at 6 h. There was no correlation between the amount if IL-5 detected by QC-RT/PCR and frequency of IL-5 mRNA+ cells by ISH. These findings also did not correlate with the degree of inflammatory responses. In conclusion: (1) greater IL-5 than IFN-γ deposition in Ag sites suggests Th2predominance in LPR; (2) lack of correlation between QC-RT/PCR and ISH findings may reflect varying mRNA content of inflammatory cells. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)1065-1075
Number of pages11
Issue number7
StatePublished - Jul 2000
Externally publishedYes


  • Cutaneous late phase reactions
  • Cytokine
  • IL-5
  • mRNA


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