TY - JOUR
T1 - Quantitative and qualitative assessment of plasma von Willebrand factor in classic Kaposi's sarcoma
AU - Hodak, Emmilia
AU - Trattner, Akiva
AU - David, Michael
AU - Kornbrot, Nurit
AU - Modan, Baruch
AU - Lurie, Hedwig
AU - Lawrie, Andrew
AU - Harrison, Paul
AU - Sandbank, Miriam
AU - Inbal, Aida
PY - 1993
Y1 - 1993
N2 - Background: von Willebrand factor (vWF) is synthesized almost exclusively by endothelial cells and is stored there as ultra-high-molecular-weight multimers. The vWF multimers that are detected in the plasma are smaller than those stored within the endothelium. In two previous studies, comprising small series of cases with classic Kaposi's sarcoma (KS), an endothelium-derived tumor, increased levels of plasma von Willebrand factor antigen (VWF:Ag, the antigenic structure) were reported, suggesting that vWF:Ag may be a marker of endothelium proliferation. Objective: Our purpose was to investigate the quantitative as well as qualitative alterations of plasma vWF in a large series of patients with classic KS at various stages of the disease. Methods: Levels of plasma vWF:Ag were studied in 38 patients with classic KS confined to the skin at various stages of the disease and compared with a control group. Thirty-three patients had active KS (i.e., with skin lesions) and five were in remission. In five patients with active KS multimeric analysis of plasma vWF was also performed. Results: The levels of vWF:Ag were significantly higher among KS patients than in the control group (n = 29, p<0.01). Levels of vWF:Ag were also significantly higher in patients with active disease as compared with those in remission (p<0.05). No correlation was found between v WF:Ag levels and the extent of KS. Analysis of the multimeric pattern of plasma vWF showed enhanced staining of all bands, particularly the intermediate and high molecular weight forms, which resemble the endothelial forms as opposed to normal circulating vWF multimers. Conclusion: Quantitative as well as qualitative alterations in plasma vWF were found in patients with KS, which may reflect the destruction or activation of endothelial cells within the lesions, vWF:Ag may serve as a marker of disease activity in classic KS; however, it is not a good marker for the extent of the disease.
AB - Background: von Willebrand factor (vWF) is synthesized almost exclusively by endothelial cells and is stored there as ultra-high-molecular-weight multimers. The vWF multimers that are detected in the plasma are smaller than those stored within the endothelium. In two previous studies, comprising small series of cases with classic Kaposi's sarcoma (KS), an endothelium-derived tumor, increased levels of plasma von Willebrand factor antigen (VWF:Ag, the antigenic structure) were reported, suggesting that vWF:Ag may be a marker of endothelium proliferation. Objective: Our purpose was to investigate the quantitative as well as qualitative alterations of plasma vWF in a large series of patients with classic KS at various stages of the disease. Methods: Levels of plasma vWF:Ag were studied in 38 patients with classic KS confined to the skin at various stages of the disease and compared with a control group. Thirty-three patients had active KS (i.e., with skin lesions) and five were in remission. In five patients with active KS multimeric analysis of plasma vWF was also performed. Results: The levels of vWF:Ag were significantly higher among KS patients than in the control group (n = 29, p<0.01). Levels of vWF:Ag were also significantly higher in patients with active disease as compared with those in remission (p<0.05). No correlation was found between v WF:Ag levels and the extent of KS. Analysis of the multimeric pattern of plasma vWF showed enhanced staining of all bands, particularly the intermediate and high molecular weight forms, which resemble the endothelial forms as opposed to normal circulating vWF multimers. Conclusion: Quantitative as well as qualitative alterations in plasma vWF were found in patients with KS, which may reflect the destruction or activation of endothelial cells within the lesions, vWF:Ag may serve as a marker of disease activity in classic KS; however, it is not a good marker for the extent of the disease.
UR - http://www.scopus.com/inward/record.url?scp=0027518240&partnerID=8YFLogxK
U2 - 10.1016/0190-9622(93)70030-W
DO - 10.1016/0190-9622(93)70030-W
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0027518240
SN - 0190-9622
VL - 28
SP - 217
EP - 221
JO - Journal of the American Academy of Dermatology
JF - Journal of the American Academy of Dermatology
IS - 2
ER -