Quantification of human serum procollagen C-proteinase enhancer (hsPCPE) glycopattern

Yael Olswang-Kuz, Boaz Liberman, Israel Weiss, Eyal Ramu, Rony Weitzen, Iris Vered, Galia Gat-Yablonski, Efrat Kessler, Yair Anikster, Shlomit Mesilaty-Gross

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Procollagen C-proteinase enhancer 1 (PCPE1), a glycoprotein secreted from differentiating osteoblast, enhances the rate-limiting step of collagen type I fibrillar formation. It is expressed and secreted by cells that produce collagen type I and has the potential to be a marker for bone pathologies. Methods: We developed an assay to quantify PCPE glycopattern based on isoelectric focusing (IEF) and detection with a bio-imaging camera (coefficient of variation within and between assays, 15% and 20%, respectively). Results: PCPE was quantified in 39 serum samples from healthy subjects (17 females and 22 males). The concentration in the serum was 305(274) ng/ml, median(IQR). The level of the PCPE isoforms and their relative distribution were altered in patients with bone disorders. Conclusions: The data generated by our system, support our hypothesis that combined data on PCPE concentration and isoforms may be useful for the diagnosis and follow-up of bone diseases. Further research, on larger cohorts of both normal subjects and patients, must be done.

Original languageEnglish
Pages (from-to)1762-1766
Number of pages5
JournalClinica Chimica Acta
Volume412
Issue number19-20
DOIs
StatePublished - 18 Sep 2011

Keywords

  • Collagen
  • Osteoblasts
  • Osteoporosis
  • Paget's disease

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