TY - JOUR
T1 - Quantification of human serum procollagen C-proteinase enhancer (hsPCPE) glycopattern
AU - Olswang-Kuz, Yael
AU - Liberman, Boaz
AU - Weiss, Israel
AU - Ramu, Eyal
AU - Weitzen, Rony
AU - Vered, Iris
AU - Gat-Yablonski, Galia
AU - Kessler, Efrat
AU - Anikster, Yair
AU - Mesilaty-Gross, Shlomit
PY - 2011/9/18
Y1 - 2011/9/18
N2 - Background: Procollagen C-proteinase enhancer 1 (PCPE1), a glycoprotein secreted from differentiating osteoblast, enhances the rate-limiting step of collagen type I fibrillar formation. It is expressed and secreted by cells that produce collagen type I and has the potential to be a marker for bone pathologies. Methods: We developed an assay to quantify PCPE glycopattern based on isoelectric focusing (IEF) and detection with a bio-imaging camera (coefficient of variation within and between assays, 15% and 20%, respectively). Results: PCPE was quantified in 39 serum samples from healthy subjects (17 females and 22 males). The concentration in the serum was 305(274) ng/ml, median(IQR). The level of the PCPE isoforms and their relative distribution were altered in patients with bone disorders. Conclusions: The data generated by our system, support our hypothesis that combined data on PCPE concentration and isoforms may be useful for the diagnosis and follow-up of bone diseases. Further research, on larger cohorts of both normal subjects and patients, must be done.
AB - Background: Procollagen C-proteinase enhancer 1 (PCPE1), a glycoprotein secreted from differentiating osteoblast, enhances the rate-limiting step of collagen type I fibrillar formation. It is expressed and secreted by cells that produce collagen type I and has the potential to be a marker for bone pathologies. Methods: We developed an assay to quantify PCPE glycopattern based on isoelectric focusing (IEF) and detection with a bio-imaging camera (coefficient of variation within and between assays, 15% and 20%, respectively). Results: PCPE was quantified in 39 serum samples from healthy subjects (17 females and 22 males). The concentration in the serum was 305(274) ng/ml, median(IQR). The level of the PCPE isoforms and their relative distribution were altered in patients with bone disorders. Conclusions: The data generated by our system, support our hypothesis that combined data on PCPE concentration and isoforms may be useful for the diagnosis and follow-up of bone diseases. Further research, on larger cohorts of both normal subjects and patients, must be done.
KW - Collagen
KW - Osteoblasts
KW - Osteoporosis
KW - Paget's disease
UR - http://www.scopus.com/inward/record.url?scp=79960638715&partnerID=8YFLogxK
U2 - 10.1016/j.cca.2011.04.030
DO - 10.1016/j.cca.2011.04.030
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AN - SCOPUS:79960638715
SN - 0009-8981
VL - 412
SP - 1762
EP - 1766
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 19-20
ER -