TY - JOUR
T1 - Qualitative monitoring of SARS-CoV-2 mRNA vaccination in humans using droplet microfluidics
AU - Broketa, Matteo
AU - Sokal, Aurélien
AU - Mor, Michael
AU - Canales-Herrerias, Pablo
AU - Perima, Angga
AU - Meola, Annalisa
AU - Fernández, Ignacio
AU - Iannascoli, Bruno
AU - Chenon, Guilhem
AU - Vandenberghe, Alexis
AU - Languille, Laetitia
AU - Michel, Marc
AU - Godeau, Bertrand
AU - Gallien, Sébastien
AU - Melica, Giovanna
AU - Backovic, Marija
AU - Rey, Felix A.
AU - Baudry, Jean
AU - Freund, Natalia T.
AU - Mahévas, Matthieu
AU - Bruhns, Pierre
N1 - Publisher Copyright:
© 2023, Broketa et al. This is an open access article published under the terms of the Creative Commons Attribution 4.0 International License.
PY - 2023/7/10
Y1 - 2023/7/10
N2 - SARS-CoV-2 mRNA vaccination generates protective B cell responses targeting the SARS-CoV-2 spike glycoprotein. Whereas anti-spike memory B cell responses are long lasting, the anti-spike humoral antibody response progressively wanes, making booster vaccinations necessary for maintaining protective immunity. Here, we qualitatively investigated the plasmablast responses by measuring from single cells within hours of sampling the affinity of their secreted antibody for the SARS-CoV-2 spike receptor binding domain (RBD) in cohorts of BNT162b2-vaccinated naive and COVID-19-recovered individuals. Using a droplet microfluidic and imaging approach, we analyzed more than 4, 000 single IgG-secreting cells, revealing high interindividual variability in affinity for RBD, with variations over 4 logs. High-affinity plasmablasts were induced by BNT162b2 vaccination against Hu-1 and Omicron RBD but disappeared quickly thereafter, whereas low-affinity plasmablasts represented more than 65% of the plasmablast response at all time points. Our droplet-based method thus proves efficient at fast and qualitative immune monitoring and should be helpful for optimization of vaccination protocols.
AB - SARS-CoV-2 mRNA vaccination generates protective B cell responses targeting the SARS-CoV-2 spike glycoprotein. Whereas anti-spike memory B cell responses are long lasting, the anti-spike humoral antibody response progressively wanes, making booster vaccinations necessary for maintaining protective immunity. Here, we qualitatively investigated the plasmablast responses by measuring from single cells within hours of sampling the affinity of their secreted antibody for the SARS-CoV-2 spike receptor binding domain (RBD) in cohorts of BNT162b2-vaccinated naive and COVID-19-recovered individuals. Using a droplet microfluidic and imaging approach, we analyzed more than 4, 000 single IgG-secreting cells, revealing high interindividual variability in affinity for RBD, with variations over 4 logs. High-affinity plasmablasts were induced by BNT162b2 vaccination against Hu-1 and Omicron RBD but disappeared quickly thereafter, whereas low-affinity plasmablasts represented more than 65% of the plasmablast response at all time points. Our droplet-based method thus proves efficient at fast and qualitative immune monitoring and should be helpful for optimization of vaccination protocols.
UR - http://www.scopus.com/inward/record.url?scp=85164250022&partnerID=8YFLogxK
U2 - 10.1172/JCI.INSIGHT.166602
DO - 10.1172/JCI.INSIGHT.166602
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C2 - 37252802
AN - SCOPUS:85164250022
SN - 2379-3708
VL - 8
JO - JCI insight
JF - JCI insight
IS - 13
M1 - e166602
ER -
