Q-space high b value diffusion MRI of hemi-crush in rat spinal cord: Evidence for spontaneous regeneration

Revital Nossin-Manor, Revital Duvdevani, Yoram Cohen

Research output: Contribution to journalArticlepeer-review

Abstract

The development of the damage following hemi-crush trauma in rat spinal cord was studied ex vivo using high b value (bmax = 1 × 107 s cm-2) q-space diffusion weighted MRI (DWI) at fives days, ten days and six weeks post-trauma. Rat spinal cord trauma, produced by hemi-crush of 15s and 60s duration, was studied. The water signal decay in these diffusion experiments was found to be non mono-exponential and was analyzed using the q-space approach. The q-space MRI parameters were compared with T1 and T2 MR images, behavioral tests and histopathological osmium staining. A very good anatomical correlation was found between the q-space MRI parameters and the osmium staining. Interestingly, we found that in the 15s hemi-crush model significant recovery was observed in both the q-space MR images and the osmium staining six weeks post-trauma. However, in the 60s hemi-crush trauma model very little recovery was observed. These results paralleled those obtained from behavioral tests demonstrating that partial spontaneous recovery seems to occur in the 15s hemi-crush spinal cord model, which should be taken in consideration when using it to evaluate new therapies.

Original languageEnglish
Pages (from-to)231-241
Number of pages11
JournalMagnetic Resonance Imaging
Volume20
Issue number3
DOIs
StatePublished - 2002

Keywords

  • DWI
  • Hemi-crush
  • MRI
  • Spinal cord
  • Trauma
  • q-Space diffusion

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