TY - JOUR
T1 - Q-space high b value diffusion MRI of hemi-crush in rat spinal cord
T2 - Evidence for spontaneous regeneration
AU - Nossin-Manor, Revital
AU - Duvdevani, Revital
AU - Cohen, Yoram
N1 - Funding Information:
Support for this project by the German Federal Ministry of Education and Research (BMBF) within the framework of the German-Israeli Project Cooperation (DIP) is gratefully acknowledged. The gradient system was purchased with a grant from the Israel Science Foundation (ISF), administered by the Israeli Academy of Sciences and Humanities. We wish to thank Dr. Y. Assaf, Mr. R. Manor and Miss R. Oz for their help.
PY - 2002
Y1 - 2002
N2 - The development of the damage following hemi-crush trauma in rat spinal cord was studied ex vivo using high b value (bmax = 1 × 107 s cm-2) q-space diffusion weighted MRI (DWI) at fives days, ten days and six weeks post-trauma. Rat spinal cord trauma, produced by hemi-crush of 15s and 60s duration, was studied. The water signal decay in these diffusion experiments was found to be non mono-exponential and was analyzed using the q-space approach. The q-space MRI parameters were compared with T1 and T2 MR images, behavioral tests and histopathological osmium staining. A very good anatomical correlation was found between the q-space MRI parameters and the osmium staining. Interestingly, we found that in the 15s hemi-crush model significant recovery was observed in both the q-space MR images and the osmium staining six weeks post-trauma. However, in the 60s hemi-crush trauma model very little recovery was observed. These results paralleled those obtained from behavioral tests demonstrating that partial spontaneous recovery seems to occur in the 15s hemi-crush spinal cord model, which should be taken in consideration when using it to evaluate new therapies.
AB - The development of the damage following hemi-crush trauma in rat spinal cord was studied ex vivo using high b value (bmax = 1 × 107 s cm-2) q-space diffusion weighted MRI (DWI) at fives days, ten days and six weeks post-trauma. Rat spinal cord trauma, produced by hemi-crush of 15s and 60s duration, was studied. The water signal decay in these diffusion experiments was found to be non mono-exponential and was analyzed using the q-space approach. The q-space MRI parameters were compared with T1 and T2 MR images, behavioral tests and histopathological osmium staining. A very good anatomical correlation was found between the q-space MRI parameters and the osmium staining. Interestingly, we found that in the 15s hemi-crush model significant recovery was observed in both the q-space MR images and the osmium staining six weeks post-trauma. However, in the 60s hemi-crush trauma model very little recovery was observed. These results paralleled those obtained from behavioral tests demonstrating that partial spontaneous recovery seems to occur in the 15s hemi-crush spinal cord model, which should be taken in consideration when using it to evaluate new therapies.
KW - DWI
KW - Hemi-crush
KW - MRI
KW - Spinal cord
KW - Trauma
KW - q-Space diffusion
UR - http://www.scopus.com/inward/record.url?scp=0036077346&partnerID=8YFLogxK
U2 - 10.1016/S0730-725X(02)00470-8
DO - 10.1016/S0730-725X(02)00470-8
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AN - SCOPUS:0036077346
VL - 20
SP - 231
EP - 241
JO - Magnetic Resonance Imaging
JF - Magnetic Resonance Imaging
SN - 0730-725X
IS - 3
ER -