Purification of a nuclease from human serum

E. J. Zöllner*, G. Seibert, H. Slor, R. K. Zahn

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The purification procedure for a nuclease from human serum is described. It includes ammonium sulfate precipitation, chromatography on DEAE-Sephadex and on Sephacryl-S 200, and preparative electrophoresis. The enzyme, purified about 2000-fold, is homogeneous in a sodium dodecyl sulfate electrophoretic system, where it has a mol. wt of 78,000. The pH optimum lies around pH 6.5; it is a sugar-nonspecific endonuclease.

Original languageEnglish
Pages (from-to)548-550
Number of pages3
Issue number6
StatePublished - Jun 1981


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