Purification of a nuclease from human serum

E. J. Zöllner; G. Seibert; H. Slor; R. K. Zahn

doi:10.1007/BF01990041

Purification of a nuclease from human serum

E. J. Zöllner^*, G. Seibert, H. Slor, R. K. Zahn

^*Corresponding author for this work

NY American Medicine Program

Research output: Contribution to journal › Article › peer-review

Abstract

The purification procedure for a nuclease from human serum is described. It includes ammonium sulfate precipitation, chromatography on DEAE-Sephadex and on Sephacryl-S 200, and preparative electrophoresis. The enzyme, purified about 2000-fold, is homogeneous in a sodium dodecyl sulfate electrophoretic system, where it has a mol. wt of 78,000. The pH optimum lies around pH 6.5; it is a sugar-nonspecific endonuclease.

Original language	English
Pages (from-to)	548-550
Number of pages	3
Journal	Experientia
Volume	37
Issue number	6
DOIs	https://doi.org/10.1007/BF01990041
State	Published - Jun 1981

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AB - The purification procedure for a nuclease from human serum is described. It includes ammonium sulfate precipitation, chromatography on DEAE-Sephadex and on Sephacryl-S 200, and preparative electrophoresis. The enzyme, purified about 2000-fold, is homogeneous in a sodium dodecyl sulfate electrophoretic system, where it has a mol. wt of 78,000. The pH optimum lies around pH 6.5; it is a sugar-nonspecific endonuclease.

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Purification of a nuclease from human serum

Abstract

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