Purification and partial characterization of vitellin from the ovaries of Parapenaeus longirostris (Crustacea, Decapoda, Penaeidae)

Moshe Tom*, Menachem Goren, Michael Ovadia

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

1. 1. The vitellin of Parapenaeus longirostris was isolated from ripe ovaries by gel filtration on Sepharose 6B followed by ion exchange chromatography DEAE-Sepharose. 2. 2. The vitellin was identified by comparative PAGE of homogenates prepared from undeveloped and from ripe ovaries as determined by histological sections. The purified vitellin showed one glycoprotein band in disc electrophoresis and one precipitation line in immunoelectrophoresis and immunodiffusion. 3. 3. SDS-PAGE of the purified substance showed two types of subunits weighing 45,000 and 66,000 daltons. 4. 4. Antiserum prepared against the purified vitellin of P. longirostris reacted with homogenates obtained from ripe ovaries of Penaeus semisulcatus.

Original languageEnglish
Pages (from-to)17-23
Number of pages7
JournalComparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
Volume87
Issue number1
DOIs
StatePublished - 1987

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