Purification and characterization of the voltage-dependent anion-selective channel protein from wheat mitochondrial membranes

Amnon Blumenthal, Kathy Kahn, Oded Beja, Esra Galun, Marco Colombini, Adina Breiman

Research output: Contribution to journalArticlepeer-review

Abstract

An approximately 29-kD protein was purified from the membrane fraction of wheat (Triticum aestivum cv Dganit) mitochondria by the utilization of standard liquid chromatography techniques. The protein, designated MmP29 for mitochondrial membrane protein having a molecular mass of approximately 29 kD, exhibited cationic properties in a buffering solution, adjusted to pH 7.5. This positive charge enabled its passage through a diethylaminoethyl column, without interaction with the positively charged matrix. Subsequently, this protein was separated from the remaining polypeptides by a preferential elution from a hydroxylapatite/celite mixed column. Reconstituted liposomes containing this protein were characterized as being permeable to 8-aminonaphthalene 1,3,6-trisulfonic acid disodium salt (Mr 445) but non-permeable to dextran fluorescein (Mr 40,000). Additionally, MmP29 was inserted into planar phospholipid membranes, and anion-selective, voltage-dependent channels were demonstrated. All of the MmP29 properties mentioned highly resemble voltage-dependent, anion-selective channel (VDAC) proteins, suggesting that MmP29 is the mitochondrial outer membrane VDAC protein of wheat.

Original languageEnglish
Pages (from-to)579-587
Number of pages9
JournalPlant Physiology
Volume101
Issue number2
DOIs
StatePublished - Feb 1993

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