Purification and characterization of pectolytic enzymes produced by virulent and hypovirulent isolates of Rhizoctonia solani Kuhn

L. Marcus*, I. Barash, B. Sneh, Y. Koltin, A. Finkler

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Four pectolytic enzymes were purified from an isolate of Rhizoctonia solani (No. 82, AG-4) virulent on a wide range of hosts (Ichielvich-Auster et al. 1985, Phyloparasilica vol. 13, 103–112). The enzymes designated as endopolygalacturonase I and II (endoPG-I and endoPG-II), pectinesterase (PE) and endopectinlyase (endoPL) have been purified to homogeneity by a single chromatographic step on a cross linked polypectate column. These enzymes were identified also in two virulent isolates of R. zeae and two virulent binucleate Rhizoctonia spp. The endoPG-I, endoPG-II and PE but not endoPL were identified in three hypovirulent isolates of R. solani and two of R. zeae. These enzymes were purified to homogeneity from R. solani (No. 521, AG-4). The molecular weight (mol.wt), pH optimum, isoelectric point (pI) and optimal temperature (T) for each enzyme were endoPG-I, mol.wt 34 000, pH 4·8, pI 6·8, T 50°C); endoPG-II, mol.wt 37 000, pH 5·4, pI 7·4, T 42°C; PE, mol.wt 26 000, pH 7·7, pI 6·2, T 48°C; and endoPL (mol.wt. 45 500, pH 8·4, pI 8·1, T 53°C.

Original languageEnglish
Pages (from-to)325-336
Number of pages12
JournalPhysiological and Molecular Plant Pathology
Volume29
Issue number3
DOIs
StatePublished - 1986

Keywords

  • PE
  • PG
  • PL
  • pectinesterase
  • pectinlyase
  • polygalacturonase

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