PTH and 1,25(OH)2Vitamin D Priming to Growth Factors Differentially Regulates the Osteoblastic Markers in MBA-15 Clonal Subpopulations

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Abstract

The functional modulation of enzymatic activities of alkaline phosphatase (ALK-P) and neutral endopeptidase (CD10/NEP) in MBA-15.4 and MBA-15.6 marrow stromal osteoblastic cells was studied. The hormonal effects of parathyroid hormone (PTH) and 1,25 (OH)2D3 combined with various growth factors (bone morphogenic protein [BMP-2 and BMP-3], TGF β and IGF-I) on these cells were monitored. The cell responses of MBA-15.4, a preosteoblastic cell, and MBA-15.6, a more mature osteoblastic cell, to the growth factors and the hormonal challenge were measured by changes of the enzymatic activities (ALK-P and CD10/NEP). The cellular response was not uniform and revealed a differential pattern.

Original languageEnglish
Pages (from-to)197-204
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume210
Issue number1
DOIs
StatePublished - 5 May 1995

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