TY - JOUR
T1 - PTH and 1,25(OH)2Vitamin D Priming to Growth Factors Differentially Regulates the Osteoblastic Markers in MBA-15 Clonal Subpopulations
AU - Benayahu, D.
AU - Fried, A.
AU - Wientroub, S.
PY - 1995/5/5
Y1 - 1995/5/5
N2 - The functional modulation of enzymatic activities of alkaline phosphatase (ALK-P) and neutral endopeptidase (CD10/NEP) in MBA-15.4 and MBA-15.6 marrow stromal osteoblastic cells was studied. The hormonal effects of parathyroid hormone (PTH) and 1,25 (OH)2D3 combined with various growth factors (bone morphogenic protein [BMP-2 and BMP-3], TGF β and IGF-I) on these cells were monitored. The cell responses of MBA-15.4, a preosteoblastic cell, and MBA-15.6, a more mature osteoblastic cell, to the growth factors and the hormonal challenge were measured by changes of the enzymatic activities (ALK-P and CD10/NEP). The cellular response was not uniform and revealed a differential pattern.
AB - The functional modulation of enzymatic activities of alkaline phosphatase (ALK-P) and neutral endopeptidase (CD10/NEP) in MBA-15.4 and MBA-15.6 marrow stromal osteoblastic cells was studied. The hormonal effects of parathyroid hormone (PTH) and 1,25 (OH)2D3 combined with various growth factors (bone morphogenic protein [BMP-2 and BMP-3], TGF β and IGF-I) on these cells were monitored. The cell responses of MBA-15.4, a preosteoblastic cell, and MBA-15.6, a more mature osteoblastic cell, to the growth factors and the hormonal challenge were measured by changes of the enzymatic activities (ALK-P and CD10/NEP). The cellular response was not uniform and revealed a differential pattern.
UR - http://www.scopus.com/inward/record.url?scp=0029008389&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1995.1646
DO - 10.1006/bbrc.1995.1646
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AN - SCOPUS:0029008389
SN - 0006-291X
VL - 210
SP - 197
EP - 204
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -