Proteome analysis in the study of the bacterial heat-shock response

Ran Rosen, Eliora Z. Ron

Research output: Contribution to journalArticlepeer-review

Abstract

In recent years, it has become clear that, in addition to the regulation of the expression of specific genes, there are global regulatory systems that control the simultaneous expression of a large number of genes in response to a variety of environmental stresses. The first of these global control systems, and of sub-stantial importance, is the heat-shock response. The heat-shock response is characterized by the induction of a large set of proteins (heat-shock proteins-HSPs) upon shifts to higher temperature and upon exposure to conditions in which proteins are denatured (i.e., alcohols, heavy metals). The heat-shock response is universal and many of the heat-shock proteins are highly conserved among species. In bacteria, the heat-shock response has been studied extensively in several Gram-positive bacteria (Bacillus subtilis) and in the Gram-negative bacteria (i.e., Escherichia coli, Agrobacterium tumefaciens). The first recognition of the molecular abundance of the bacterial heat-shock proteins look place with the introduction of high-resolution two-dimensional polyacrylamide gels (2D gels) to analyze complex mixtures of cellular proteins. Two-dimensional gels, followed by mass spectrometry, were used to define the heat-shock stimulons in several bacteria, and to study the regulatory elements that control the heat-shock response. Here, we review the heat-shock response and its regulation in bacteria. The review will emphasize The use ofproteome analysis in the study of this response, and will point out those open questions that can be investigated with proteomics, including mass spectrometry techniques.

Original languageEnglish
Pages (from-to)244-265
Number of pages22
JournalMass Spectrometry Reviews
Volume21
Issue number4
DOIs
StatePublished - Jul 2002

Keywords

  • 2D gel electrophoresis
  • Agrobacterium tumefaciens
  • Bacillus subtilis
  • Escherchia coli
  • Post-translational modifications
  • Stress response

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