Proteinaceous microspheres for targeted RNA delivery prepared by an ultrasonic emulsification method

Ulyana Shimanovich, Dror Eliaz, Sally Zigdon, Vadim Volkov, Adva Aizer, Artur Cavaco-Paulo, Shulamit Michaeli, Yaron Shav-Tal, Aharon Gedanken*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

In the present work we used sonochemically prepared proteinaceous BSA spheres as a novel RNA-delivery system. The preparation of RNA-loaded BSA spheres was accomplished using an environmental friendly method termed the "ultrasonic emulsification method". It was demonstrated that ultrasonic waves do not cause the RNA chains to degrade and the RNA molecules remain untouched. The BSA-RNA complex was successfully introduced into mammalian (human) U2OS osteosarcoma cells and Trypanosoma brucei parasites. Using PVA coating of the RNA-BSA spheres we have achieved a significant increase in the number of microspheres penetrating mammalian cells. The mechanism of RNA encapsulation and the structure of the RNA-BSA complex are reported.

Original languageEnglish
Pages (from-to)82-90
Number of pages9
JournalJournal of Materials Chemistry B
Volume1
Issue number1
DOIs
StatePublished - 7 Jan 2013
Externally publishedYes

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