Protein-protein-interactions in a multiplexed, miniaturized format a functional analysis of Rho GTPase activation and inhibition

Michael Schmohl; Stefanie Rimmele; Oliver Pötz; Yoel Kloog; Peter Gierschik; Thomas O. Joos; Nicole Schneiderhan-Marra

doi:10.1002/pmic.200900597

Protein-protein-interactions in a multiplexed, miniaturized format a functional analysis of Rho GTPase activation and inhibition

Michael Schmohl, Stefanie Rimmele, Oliver Pötz, Yoel Kloog, Peter Gierschik, Thomas O. Joos, Nicole Schneiderhan-Marra^*

^*Corresponding author for this work

Department of Neurobiology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A miniaturized, bead-based protein-protein-interaction assay was developed to study the interaction of Rho GTPases with regulatory proteins. The setup, which uses only minute amounts of sample, was used to analyze small molecules that inhibit the interaction between Rho GTPases and RhoGDIα. Prenylcysteine analogues and the replacement of GDP by nonhydrolysable GTP analogues prevented the formation of Rho GTPase-RhoGDIα complexes in a concentration-dependent manner.

Original language	English
Pages (from-to)	1716-1720
Number of pages	5
Journal	Proteomics
Volume	10
Issue number	8
DOIs	https://doi.org/10.1002/pmic.200900597
State	Published - Apr 2010

Keywords

Automation
Bead-based assay
Prenylcysteine analogues
Protein arrays Rac
RhoGDI

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10.1002/pmic.200900597

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abstract = "A miniaturized, bead-based protein-protein-interaction assay was developed to study the interaction of Rho GTPases with regulatory proteins. The setup, which uses only minute amounts of sample, was used to analyze small molecules that inhibit the interaction between Rho GTPases and RhoGDIα. Prenylcysteine analogues and the replacement of GDP by nonhydrolysable GTP analogues prevented the formation of Rho GTPase-RhoGDIα complexes in a concentration-dependent manner.",

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AU - Kloog, Yoel

AU - Gierschik, Peter

AU - Joos, Thomas O.

AU - Schneiderhan-Marra, Nicole

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KW - Prenylcysteine analogues

KW - Protein arrays Rac

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Protein-protein-interactions in a multiplexed, miniaturized format a functional analysis of Rho GTPase activation and inhibition

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Keywords

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