TY - JOUR
T1 - Protein kinase C is present in human sperm
T2 - Possible role in flagellar motility
AU - Rotem, Ronit
AU - Paz, Gedalia F.
AU - Homonnai, Zvi T.
AU - Kalina, Moshe
AU - Naor, Zvi
PY - 1990/9
Y1 - 1990/9
N2 - We report the presence of protein kinase C (PKC) in ejaculated human sperm as revealed by enzymatic activity assay and indirect immunohistochemistry. PKC is localized in the equatorial segment and in the principal piece of the tail. Addition of phorbol 12-myristate 13-acetate resulted in increased flagellar motility that was blocked by known PKC inhibitors such as sphingosine, staurosporine, and 1-(5-isoquinoylinylsulfonyl)-2-methylpiperazine. A very good correlation (r = 0.9, P < 0.001) was found between the percentage of PKC-stained sperm cells and motility. We propose that PKC is involved in the regulation of flagellar motility in human sperm.
AB - We report the presence of protein kinase C (PKC) in ejaculated human sperm as revealed by enzymatic activity assay and indirect immunohistochemistry. PKC is localized in the equatorial segment and in the principal piece of the tail. Addition of phorbol 12-myristate 13-acetate resulted in increased flagellar motility that was blocked by known PKC inhibitors such as sphingosine, staurosporine, and 1-(5-isoquinoylinylsulfonyl)-2-methylpiperazine. A very good correlation (r = 0.9, P < 0.001) was found between the percentage of PKC-stained sperm cells and motility. We propose that PKC is involved in the regulation of flagellar motility in human sperm.
KW - Immunohistochemistry
KW - Phorbol ester
UR - http://www.scopus.com/inward/record.url?scp=0025144748&partnerID=8YFLogxK
U2 - 10.1073/pnas.87.18.7305
DO - 10.1073/pnas.87.18.7305
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C2 - 2402510
AN - SCOPUS:0025144748
SN - 0027-8424
VL - 87
SP - 7305
EP - 7308
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 18
ER -