Protein kinase C is present in human sperm: Possible role in flagellar motility

Ronit Rotem*, Gedalia F. Paz, Zvi T. Homonnai, Moshe Kalina, Zvi Naor

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

93 Scopus citations


We report the presence of protein kinase C (PKC) in ejaculated human sperm as revealed by enzymatic activity assay and indirect immunohistochemistry. PKC is localized in the equatorial segment and in the principal piece of the tail. Addition of phorbol 12-myristate 13-acetate resulted in increased flagellar motility that was blocked by known PKC inhibitors such as sphingosine, staurosporine, and 1-(5-isoquinoylinylsulfonyl)-2-methylpiperazine. A very good correlation (r = 0.9, P < 0.001) was found between the percentage of PKC-stained sperm cells and motility. We propose that PKC is involved in the regulation of flagellar motility in human sperm.

Original languageEnglish
Pages (from-to)7305-7308
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number18
StatePublished - Sep 1990


  • Immunohistochemistry
  • Phorbol ester


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