Protective effects of β-sheet breaker α/β-hybrid peptide against amyloid β-induced neuronal apoptosis in vitro

Sourav Kumar, Ashim Paul, Sourav Kalita, Anup Kumar Ghosh, Bhubaneswar Mandal*, Amal Chandra Mondal

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Alzheimer's disease is most common neurodegenerative disorder and is characterized by increased production of soluble amyloid-β oligomers, the main toxic species predominantly formed from aggregation of monomeric amyloid-β (Aβ). Increased production of Aβ invokes a cascade of oxidative damages to neurons and eventually leads to neuronal death. This study was aimed to investigate the neuroprotective effects of a β-sheet breaker α/β-hybrid peptide (BSBHp) and the underlying mechanisms against Aβ40-induced neurotoxicity in human neuroblastoma SH-SY5Y cells. Cells were pretreated with the peptide Aβ40 to induce neurotoxicity. Assays for cell viability, cell membrane damage, cellular apoptosis, generation of reactive oxygen species (ROS), intracellular free Ca2+, and key apoptotic protein levels were performed in vitro. Our results showed that pretreatment with BSBHp significantly attenuates Aβ40-induced toxicity by retaining cell viability, suppressing generation of ROS, Ca2+ levels, and effectively protects neuronal apoptosis by suppressing pro-apoptotic protein Bax and up-regulating antiapoptotic protein Bcl-2. These results suggest that α/β-hybrid peptide has neuroprotective effects against Aβ40-induced oxidative stress, which might be a potential therapeutic agent for treating or preventing neurodegenerative diseases.

Original languageEnglish
Pages (from-to)888-900
Number of pages13
JournalChemical Biology and Drug Design
Issue number6
StatePublished - 1 Jun 2017
Externally publishedYes


  • Alzheimer's disease
  • SH-SY5Y cell
  • amyloid-β peptide
  • apoptosis
  • human cerebrospinal fluid
  • neuroprotection
  • β-sheet breaker α/β-hybrid peptide


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