TY - JOUR
T1 - Protease nexin-1 complexes and inhibits T cell serine proteinase-1
AU - Gurwitz, David
AU - Simon, Markus M.
AU - Fruth, Ulli
AU - Cunningham, Dennis D.
N1 - Funding Information:
ACKNOWLEDGMENT We thank A.L. Lau and S.L. Wagner for purified PN-1, and M. Straussa nd G. Schreiber for helpful suggestions.T his work was supportedb y NM Grant GM-31609 (D.D.C.) and by the DeutscheF orschungsgemeinschaGft rant Si 214/5-4/5 (M.M.S.).
PY - 1989/5/30
Y1 - 1989/5/30
N2 - The T cell serine proteinase-1 (TSP-1) which most probably is involved in cell killing by cytotoxic T cells is inhibited by protease nexin-1 (PN-1), an extravascular serine protease inhibitor. The inhibition is irreversible and correlates with formation of SDS-stable complexes between the two proteins. Two distinct species of complexes (91 and 122 kDa) are observed upon SDS-PAGE analysis of the reacted proteins, indicating that PN-1 is capable of complexing and inhibiting both subunits of the homodimeric TSP-1 molecule. Heparin (2 ug/ml) increases the association rate constant from 4.2×104 M-1 sec-1 to 4.8×105 M-1 sec-1. These observations suggest that PN-1 may function as a major extravascular inhibitor of TSP-1 released from cytotoxic T lymphocytes.
AB - The T cell serine proteinase-1 (TSP-1) which most probably is involved in cell killing by cytotoxic T cells is inhibited by protease nexin-1 (PN-1), an extravascular serine protease inhibitor. The inhibition is irreversible and correlates with formation of SDS-stable complexes between the two proteins. Two distinct species of complexes (91 and 122 kDa) are observed upon SDS-PAGE analysis of the reacted proteins, indicating that PN-1 is capable of complexing and inhibiting both subunits of the homodimeric TSP-1 molecule. Heparin (2 ug/ml) increases the association rate constant from 4.2×104 M-1 sec-1 to 4.8×105 M-1 sec-1. These observations suggest that PN-1 may function as a major extravascular inhibitor of TSP-1 released from cytotoxic T lymphocytes.
UR - https://www.scopus.com/pages/publications/0024384736
U2 - 10.1016/0006-291X(89)91596-9
DO - 10.1016/0006-291X(89)91596-9
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AN - SCOPUS:0024384736
SN - 0006-291X
VL - 161
SP - 300
EP - 304
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -