Prospective randomized comparison of two embryo culture systems: P1 medium by irvine scientific and the cook IVF medium

Dalit Ben-Yosef, Ami Amit, Foad Azem, Tamar Schwartz, Tania Cohen, Nava Mei-Raz, Ariela Carmon, Joseph B. Lessing, Yuval Yaron

Research output: Contribution to journalArticlepeer-review


Purpose: To compare the efficacy of two commercially available in vitro fertilization (IVF) and embryo culture media systems: the glucose-free P1 Medium supplemented with 20% synthetic serum substitute (SSS) (Irvine Scientific), and the Cook IVF Medium (Cook, Australia). Methods: A prospective randomized study. Medical center-based IVF Unit affiliated to the Faculty of Medicine of Tel Aviv University. IVF patients were randomly assigned to either P1 Medium supplemented with 20% SSS (182 patients, 196 cycles) or Cook Medium (167 patients, 179 cycles). Results: Fertilization rates were similar with both media (52.3 ± 26.1 and 53.8 ± 27.6, respectively). Likewise, no difference was found in morphological characteristics and grading of cultured embryos. However, a significantly higher proportion of the embryos incubated in the P1 Medium reached the four-cell stage on day 2 or the 6-cell stage on day 3 postfertilization, compared to those incubated in Cook Medium (54.3% vs. 41.9%, p<0.0001). Clinical pregnancy and delivery rates were improved when oocytes and embryos were cultured in P1 Medium. Finally, Implantation rate was significantly higher in the P1 Medium Group (9.9% vs. 6%, respectively). Conclusions: Our results suggest that the P1 Medium may be associated with a higher embryo cleavage rate and improved implantation rates compared to the Cook IVF Medium.

Original languageEnglish
Pages (from-to)291-295
Number of pages5
JournalJournal of Assisted Reproduction and Genetics
Issue number8
StatePublished - Aug 2004
Externally publishedYes


  • IVF
  • culture media
  • implantation rate
  • preimplantation embryo development


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