Prophage excision activates listeria competence genes that promote phagosomal escape and virulence

Lev Rabinovich, Nadejda Sigal, Ilya Borovok, Ran Nir-Paz, Anat A. Herskovits

Research output: Contribution to journalArticlepeer-review

Abstract

The DNA uptake competence (Com) system of the intracellular bacterial pathogen Listeria monocytogenes is considered nonfunctional. There are no known conditions for DNA transformation, and the Com master activator gene, comK, is interrupted by a temperate prophage. Here, we show that the L. monocytogenes Com system is required during infection to promote bacterial escape from macrophage phagosomes in a manner that is independent of DNA uptake. Further, we find that regulation of the Com system relies on the formation of a functional comK gene via prophage excision. Prophage excision is specifically induced during intracellular growth, primarily within phagosomes, yet, in contrast to classic prophage induction, progeny virions are not produced. This study presents the characterization of an active prophage that serves as a genetic switch to modulate the virulence of its bacterial host in the course of infection.

Original languageEnglish
Pages (from-to)792-802
Number of pages11
JournalCell
Volume150
Issue number4
DOIs
StatePublished - 17 Aug 2012

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