Properties of solubilized prostaglandin synthetase from sheep vesicular glands

A. Raz*, Y. Kloog, E. Perel, R. Kenig-Wakshal

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Prostaglandin synthetase activity associated with the microsomal fraction from sheep vesicular glands has been solubilized by treatment with the non-ionic detergents Tween 20, Lubrol Px and Lubrol Wx. Approx.8 fold purification from microsomes is obtained and over 90% of the activity is recovered in the detergent solubilized fraction. The solubilized synthetase activity is stable at pH 5.0 but is gradually lost at pH 8.0; it is also heat and acid labile. The relative amounts of prostaglandins E2, D2 and F formed by the microsomal-bound synthetase and by the solubilized synthetase are similar. Also similar are the pH optima (7.9-8.5) of the two synthetase preparations. The solubilization process appears to yield a fully active enzymatic preparation which could be employed for further purification and characterization of the prostaglandin synthetase complex.

Original languageEnglish
Pages (from-to)951-958
Number of pages8
JournalLife Sciences
Issue number6
StatePublished - 15 Sep 1975


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