Properties of solubilized prostaglandin synthetase from sheep vesicular glands

A. Raz; Y. Kloog; E. Perel; R. Kenig-Wakshal

doi:10.1016/0024-3205(75)90448-8

Properties of solubilized prostaglandin synthetase from sheep vesicular glands

A. Raz^*, Y. Kloog, E. Perel, R. Kenig-Wakshal

^*Corresponding author for this work

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Prostaglandin synthetase activity associated with the microsomal fraction from sheep vesicular glands has been solubilized by treatment with the non-ionic detergents Tween 20, Lubrol Px and Lubrol Wx. Approx.8 fold purification from microsomes is obtained and over 90% of the activity is recovered in the detergent solubilized fraction. The solubilized synthetase activity is stable at pH 5.0 but is gradually lost at pH 8.0; it is also heat and acid labile. The relative amounts of prostaglandins E₂, D₂ and F_2α formed by the microsomal-bound synthetase and by the solubilized synthetase are similar. Also similar are the pH optima (7.9-8.5) of the two synthetase preparations. The solubilization process appears to yield a fully active enzymatic preparation which could be employed for further purification and characterization of the prostaglandin synthetase complex.

Original language	English
Pages (from-to)	951-958
Number of pages	8
Journal	Life Sciences
Volume	17
Issue number	6
DOIs	https://doi.org/10.1016/0024-3205(75)90448-8
State	Published - 15 Sep 1975

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AU - Raz, A.

AU - Kloog, Y.

AU - Perel, E.

AU - Kenig-Wakshal, R.

PY - 1975/9/15

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N2 - Prostaglandin synthetase activity associated with the microsomal fraction from sheep vesicular glands has been solubilized by treatment with the non-ionic detergents Tween 20, Lubrol Px and Lubrol Wx. Approx.8 fold purification from microsomes is obtained and over 90% of the activity is recovered in the detergent solubilized fraction. The solubilized synthetase activity is stable at pH 5.0 but is gradually lost at pH 8.0; it is also heat and acid labile. The relative amounts of prostaglandins E2, D2 and F2α formed by the microsomal-bound synthetase and by the solubilized synthetase are similar. Also similar are the pH optima (7.9-8.5) of the two synthetase preparations. The solubilization process appears to yield a fully active enzymatic preparation which could be employed for further purification and characterization of the prostaglandin synthetase complex.

AB - Prostaglandin synthetase activity associated with the microsomal fraction from sheep vesicular glands has been solubilized by treatment with the non-ionic detergents Tween 20, Lubrol Px and Lubrol Wx. Approx.8 fold purification from microsomes is obtained and over 90% of the activity is recovered in the detergent solubilized fraction. The solubilized synthetase activity is stable at pH 5.0 but is gradually lost at pH 8.0; it is also heat and acid labile. The relative amounts of prostaglandins E2, D2 and F2α formed by the microsomal-bound synthetase and by the solubilized synthetase are similar. Also similar are the pH optima (7.9-8.5) of the two synthetase preparations. The solubilization process appears to yield a fully active enzymatic preparation which could be employed for further purification and characterization of the prostaglandin synthetase complex.

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Properties of solubilized prostaglandin synthetase from sheep vesicular glands

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