@article{4510da824f00468f9255aa9046893805,
title = "Properties of aflatoxin-DNA adducts formed by photoactivation and characterization of the major photoadduct as aflatoxin-N7-guanine",
abstract = "DNA adducts were formed by microsomal and photoactivation, using nick-translated DNA labelled with 14C in each of the DNA bases [3H]AFB1 and [3H] AFB2. DNA adducts were analysed by HPLC of DNA hydrolysates, and were characterized as double labelled peaks with specific retention times. The only AF-DNA adducts which were detected in signincant amounts were guanine adducts, irrespective of the type of aflatoxin used or the mode of its activation. No stable adduct with adenine, cytcshe or thymine was detected. UV spectra, proton NMR spectroscopy and mass spectrometry are consistent with the notion that the major AFB1-DNA photoadduct is the N7-guanine adduct. This report provides direct evidence for the existence of ahtoxin photoadducts formed on DNA.",
author = "Gad Shaulsky and Johnson, {Robert L.} and Shockcor, {John P.} and Taylor, {Lester C.E.} and Stark, {Avishay Abraham}",
note = "Funding Information: We wish to thank Professor G.N.Wogan, Center for Environmental Health Sciences, MIT, Cambridge, MA, for his encouragement, Dr J.D.Groopman, School of Public Health, Boston University, for the AFB,-N7-Gua standard. Professor A.L.Demain, Department of Biology, and Dr D.F.Liberman, Environmental Medical Service, MIT, Cambridge, MA, for continued interest and support, Dr A.Abu-Shakra, Cellular and Genetic Toxicology Branch, NIEHS, NC, for help in preparative HPLC of the photoadduct, and Professor B.Z Fuchs, Department of Chemistry, Tel-Aviv University, Israel, for the photoreactor. This work was supported by Grant No. 3366/84 of the United States - Israel Binational Science Foundation",
year = "1990",
month = apr,
doi = "10.1093/carcin/11.4.519",
language = "אנגלית",
volume = "11",
pages = "519--527",
journal = "Carcinogenesis",
issn = "0143-3334",
publisher = "Oxford University Press",
number = "4",
}
