TY - JOUR
T1 - Propagation of lewis rat encephalitogenic T cell lines
T2 - T-cell-growth-factor is superior to recombinant IL-2
AU - Mor, Felix
AU - Cohen, Irun R.
N1 - Funding Information:
Prof. Irun R. Cohen is the incumbent of the Mauerberger Chair in Immunology and the Director of the Robert Koch-Minerva Center for Research in Autoimmune Diseases. This work was supported by the Minerva Center for Research in Autoimmune Diseases.
PY - 2002
Y1 - 2002
N2 - This study was designed to test the process of selecting encephalitogenic T cell lines in the Lewis rat using recombinant human IL-2 (rhIL-2) in comparison to TCGF. The lines were tested for growth, antigen induced proliferation, cytokine production, V-beta 8.2 expression and pathogenicity. We now report that rhIL-2 and TCGF were equally effective in supporting short-term pathogenic T-cell lines with similar proportion of V-beta 8.2 usage. For the maintenance of long term lines, however, TCGF was superior to IL-2. The concentration of rhIL-2 influenced the cultures: 10 units/ml led to more T-cell proliferation than either 2 or 50 units/ml. However, 50 units/ml of IL-2 led to enhanced Th1 polarization. Thus, the type and concentration of growth factors can influence both the propagation of T cells and their phenotype.
AB - This study was designed to test the process of selecting encephalitogenic T cell lines in the Lewis rat using recombinant human IL-2 (rhIL-2) in comparison to TCGF. The lines were tested for growth, antigen induced proliferation, cytokine production, V-beta 8.2 expression and pathogenicity. We now report that rhIL-2 and TCGF were equally effective in supporting short-term pathogenic T-cell lines with similar proportion of V-beta 8.2 usage. For the maintenance of long term lines, however, TCGF was superior to IL-2. The concentration of rhIL-2 influenced the cultures: 10 units/ml led to more T-cell proliferation than either 2 or 50 units/ml. However, 50 units/ml of IL-2 led to enhanced Th1 polarization. Thus, the type and concentration of growth factors can influence both the propagation of T cells and their phenotype.
KW - EAE
KW - Interleukin-2
KW - T cell lines
KW - TCGF
UR - http://www.scopus.com/inward/record.url?scp=0036190574&partnerID=8YFLogxK
U2 - 10.1016/S0165-5728(01)00477-5
DO - 10.1016/S0165-5728(01)00477-5
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C2 - 11880152
AN - SCOPUS:0036190574
SN - 0165-5728
VL - 123
SP - 76
EP - 82
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1-2
ER -