Propagation of lewis rat encephalitogenic T cell lines: T-cell-growth-factor is superior to recombinant IL-2

Felix Mor*, Irun R. Cohen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

This study was designed to test the process of selecting encephalitogenic T cell lines in the Lewis rat using recombinant human IL-2 (rhIL-2) in comparison to TCGF. The lines were tested for growth, antigen induced proliferation, cytokine production, V-beta 8.2 expression and pathogenicity. We now report that rhIL-2 and TCGF were equally effective in supporting short-term pathogenic T-cell lines with similar proportion of V-beta 8.2 usage. For the maintenance of long term lines, however, TCGF was superior to IL-2. The concentration of rhIL-2 influenced the cultures: 10 units/ml led to more T-cell proliferation than either 2 or 50 units/ml. However, 50 units/ml of IL-2 led to enhanced Th1 polarization. Thus, the type and concentration of growth factors can influence both the propagation of T cells and their phenotype.

Original languageEnglish
Pages (from-to)76-82
Number of pages7
JournalJournal of Neuroimmunology
Volume123
Issue number1-2
DOIs
StatePublished - 2002
Externally publishedYes

Funding

FundersFunder number
Minerva Center for Research in Autoimmune Diseases

    Keywords

    • EAE
    • Interleukin-2
    • T cell lines
    • TCGF

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