Prolonged infusion of epinephrine down-regulates expression of the fatty acid synthase gene in adipocytes

Lotan Shilo, Jane H. Chin, Salman Azhar, Brian B. Hoffman

Research output: Contribution to journalArticlepeer-review

Abstract

Fatty acid synthase is a key enzyme in the de novo synthesis of fatty acids. Expression of fatty acid synthase mRNA in adipocytes is inhibited by beta adrenergic agonists. We wondered if prolonged exposure to high concentrations of catecholamines might inhibit expression of the fatty acid synthase gene in fat cells. In this study we investigated the effects of adrenergic stimulation on the regulation of fatty acid synthase in fat cells. Rats were infused continuously with epinephrine (60 μg/kg/hr) or vehicle for 48 h and fat pads were isolated. The infusion of epinephrine down-regulated expression of the fatty acid synthase mRNA as well as decreasing enzyme activity. Both epinephrine and isoproterenol inhibited fatty acid synthase mRNA expression when adipocytes were activated in vitro. These effects were blocked by the β adrenergic antagonist propranolol. In contrast, incubation with the adenosine receptor agonist phenylisopropyl-adenosine which decreases cAMP accumulation in fat cells, caused an increase in accumulation of fatty acid synthase mRNA. These results indicate that prolonged exposure to catecholamines, acting via β adrenergic receptors, inhibit expression of the fatty acid synthase gene possibly by increasing intracellular concentrations of cAMP.

Original languageEnglish
Pages (from-to)439-444
Number of pages6
JournalEndocrine
Volume3
Issue number6
DOIs
StatePublished - Jun 1995
Externally publishedYes

Keywords

  • adenosine
  • adipocytes
  • beta adrenergic
  • fatty acid synthase
  • gene expression
  • lipolysis

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