Prolongation of the lag time preceding peroxidation of serum lipids: A measure of antioxidant capacity

Ilya Pinchuk*, Dov Lichtenberg

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Antioxidants inhibit oxidation processes and by this affect many biological processes. This, in turn, promotes continuing efforts to synthesize new efficient antioxidants and discover compounds of natural origin capable of preventing peroxidation. Although many assays have been developed to evaluate antioxidants, the search for improved protocols is still actual. The presented protocol is based on the effect of antioxidant on the kinetics of peroxidation of lipids in human blood serum. Specifically, we evaluate the capacity of antioxidant by the relative prolongation of lag phase (delay) of copper-induced peroxidation of lipids in unfractionated serum. The main advantage of the assay is that it implements inhibition of peroxidation in physiologically relevant system. We propose expressing the results of the assay either in terms of the relative prolongation of the lag per 1 μM of antioxidant or as the concentration of antioxidant required to double the lag. To allow for comparing the results with those of other assays, these results may be normalized and expressed in terms of the unitless "TROLOX equivalents".

Original languageEnglish
Title of host publicationAdvanced Protocols in Oxidative Stress III
PublisherSpringer New York
Number of pages10
ISBN (Electronic)9781493914418
ISBN (Print)9781493914401
StatePublished - 16 Oct 2014


  • Antioxidants
  • Assays
  • Oxidative stress
  • Serum lipid peroxidation


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