TY - JOUR
T1 - Programmable comprehensive controller for multi-color 3D confocal spinning-disk image scanning microscope
AU - Flaxer, Eli
AU - Bram, Lanna
AU - Flaxer, Alona
AU - Roichman, Yael
AU - Ebenstein, Yuval
N1 - Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2024/3
Y1 - 2024/3
N2 - This paper introduces a compact, portable, and highly accurate triggering control system for a 3D confocal spinning-disk image scanning microscope (CSD-ISM). Building upon on our previously published research, we expanded the hardware of the controller and synchronized it with a sub-micron translator which scans the object in the z-direction. As well as expanding the hardware, the software also was extended from previously published work similarly as it is stated for hardware while allowing full control over the 3D movement. We showed a clear and smooth 3D image made up of a collection of 2D images at different heights.
AB - This paper introduces a compact, portable, and highly accurate triggering control system for a 3D confocal spinning-disk image scanning microscope (CSD-ISM). Building upon on our previously published research, we expanded the hardware of the controller and synchronized it with a sub-micron translator which scans the object in the z-direction. As well as expanding the hardware, the software also was extended from previously published work similarly as it is stated for hardware while allowing full control over the 3D movement. We showed a clear and smooth 3D image made up of a collection of 2D images at different heights.
KW - Fluorescence microscopy
KW - Image scanning microscope
KW - Spinning-disk microscope
UR - http://www.scopus.com/inward/record.url?scp=85179585352&partnerID=8YFLogxK
U2 - 10.1016/j.ultramic.2023.113888
DO - 10.1016/j.ultramic.2023.113888
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C2 - 38086290
AN - SCOPUS:85179585352
SN - 0304-3991
VL - 257
JO - Ultramicroscopy
JF - Ultramicroscopy
M1 - 113888
ER -