@inbook{ebe1d67f2af7489699190b80248ceeca,
title = "Profiling of Secreted Type 3 Secretion System Substrates by Salmonella enterica",
abstract = "Many of Salmonella enterica virulence-associated phenotypes, including its ability to manipulate various host pathways are mediated by translocation of specific effector proteins via type 3 secretion systems (T3SSs) into the host cell. Culturing Salmonella under a defined set of stimulating conditions in vitro can mimic the physiological signals Salmonella senses during the infection and results in the secretion of these effectors into the growth medium. Here we describe a Salmonella secretion assay to identify and quantify protein substrates secreted by T3SS-1 and demonstrate how this method can be utilized to study the secretion of T3SS-1 effectors and flagellum components in different genetic backgrounds or under varying growth conditions.",
keywords = "Effectors, Flagella, SPI-1, SPI-2, Salmonella, Secretion assay, Type 3 secretion system (T3SS)",
author = "Rivka Shem-Tov and Ohad Gal-Mor",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-1971-1_5",
language = "אנגלית",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "47--54",
booktitle = "Methods in Molecular Biology",
}