Profiling of Secreted Type 3 Secretion System Substrates by Salmonella enterica

Rivka Shem-Tov, Ohad Gal-Mor*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations


Many of Salmonella enterica virulence-associated phenotypes, including its ability to manipulate various host pathways are mediated by translocation of specific effector proteins via type 3 secretion systems (T3SSs) into the host cell. Culturing Salmonella under a defined set of stimulating conditions in vitro can mimic the physiological signals Salmonella senses during the infection and results in the secretion of these effectors into the growth medium. Here we describe a Salmonella secretion assay to identify and quantify protein substrates secreted by T3SS-1 and demonstrate how this method can be utilized to study the secretion of T3SS-1 effectors and flagellum components in different genetic backgrounds or under varying growth conditions.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages8
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


FundersFunder number
Volks-wagenStiftung Research Cooperation Lower Saxony–Israel2616/18
German-Israeli Foundation for Scientific Research and DevelopmentA128055


    • Effectors
    • Flagella
    • SPI-1
    • SPI-2
    • Salmonella
    • Secretion assay
    • Type 3 secretion system (T3SS)


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