Abstract
Western blot analysis was used to investigate protein kinase C (PKC) profile of rat eggs. The presence of eight PKC isozymes was demonstrated: conventional PKC α, β and γ; novel PKC δ, ε and μ; atypical PKC ζ and λ. PKC α was detected by RT-PCR as well. PKC translocation from the cytosol to the plasma membrane served as a marker for enzyme activation. Immunofluorescence confocal microscopy demonstrated a relatively uniform distribution of PKC α, βI, and βII throughout the cytosol of metaphase II arrested eggs. PKC accumulation at the plasma membrane was detected 5 min after exposure to 12-O-tetradecanoyl phorbol-13-acetate and increased with time, thus demonstrating activation of these PKCs.
Original language | English |
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Pages (from-to) | 415-418 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 431 |
Issue number | 3 |
DOIs | |
State | Published - 24 Jul 1998 |
Keywords
- Cortical granule
- Egg activation
- Fertilization
- Protein kinase C
- Rat egg