Profile of gene expression regulated by induced p53: Connection to the TGF-β family

Karuppiah Kannan, Ninette Amariglio, Gideon Rechavi, David Givol*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The transcription regulatory function of p53 was analyzed by using two inducible p53 systems in the human lung cancer cell line H1299. cDNA probes derived from RNA harvested 12 h after p53 induction were used to probe filters containing cDNA arrays. Over 20 genes were found to be significantly induced or suppressed by p53. The induced genes can be classified mainly as cell cycle inhibitors like p21waf, GADD45, apoptosis-related genes like Fas/APO1 and PIG3 or DNA repair genes like DDB2, DNA ligase and G/T mismatch DNA glycosylase. The suppressed genes include mainly cell cycle regulators like cyclin B1, cyclin H and kinases like c-abl, CLK1 and others. The most notable induced gene was MIC-1, encoding a TGF-β-related secretory protein, suggesting a potential paracrine component for p53 growth suppression. (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)77-82
Number of pages6
JournalFEBS Letters
Issue number1
StatePublished - 17 Mar 2000
Externally publishedYes


  • DNA array
  • DNA repair
  • MIC-1
  • Transactivation
  • Transrepression
  • p53


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