@article{7c7d802003fd4b8783e6409662bf9df1,
title = "Production of F(ab′)2 from Monoclonal and Polyclonal Antibodies",
abstract = "Antibodies are widely used in therapeutic, diagnostic, and research applications, and antibody derivatives such as F(ab′)2 fragments are used when only a particular antibody region is required. F(ab′)2 can be produced through antibody engineering, but some applications require F(ab′)2 produced from an original formulated antibody or directly from a polyclonal antibody pool. The cysteine protease immunoglobulin-degrading enzyme (IdeS) from Streptococcus pyogenes digests immunoglobulin G (IgG) specifically and efficiently to produce F(ab′)2. Here we detail the production and purification of recombinant IdeS; its utilization to digest monoclonal or polyclonal antibodies to F(ab′)2 fragments; and F(ab′)2 purification through consecutive affinity chromatography steps. The resultant F(ab′)2 exhibit high purity, retain antigen-binding functionality, and are readily utilizable in various downstream applications.",
keywords = "IdeS, mAbs, monoclonal antibody, polyclonal antibodies",
author = "Shai Rosenstein and Anna Vaisman-Mentesh and Limor Levy and Aya Kigel and Yael Dror and Yariv Wine",
note = "Publisher Copyright: {\textcopyright} 2020 John Wiley & Sons, Inc.",
year = "2020",
month = jun,
day = "1",
doi = "10.1002/cpmb.119",
language = "אנגלית",
volume = "131",
journal = "Current Protocols in Molecular Biology",
issn = "1934-3639",
publisher = "John Wiley & Sons Inc.",
number = "1",
}