Processing of asparagine-linked saccharides in Mucor rouxii

Gerardo Z. Lederkremer, Armando J. Parodi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Asparagine-linked Glc1Man9GlcNAc2, Glc1Man8GlcNAc2 and Glc1Man7GlcNAc2 were detected in mycelial-form cells of the dimorphic fungus Mucor rouxii inculbated with [U-14C]glucose for 3 min. The oligosaccharides were absent from glycoproteins isolated from cells chased for 15 min with the unlabed monosaccharide. This was due to deglucosylation of the oligosaccharides and not to further addition of mannose residues to them. The half-lives of the glucosylated compounds were much shorter, therefore, in M. rouxii than in other eucaryotic cells. Further processing of N-linked saccharides led to the synthesis of mannan-like glycoproteins, some of whch contained methyl groups in position 3 or the mannose residues. Methylation occurred only at the non-reducing ends and prevented further elongation of the branches.

Original languageEnglish
Pages (from-to)363-369
Number of pages7
JournalBiochimica et Biophysica Acta - General Subjects
Issue number2
StatePublished - 19 Nov 1986
Externally publishedYes


  • (Mucor)
  • Asparagine
  • Glucosylation
  • Glycoprotein processing


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