Procarbazine effects on spermatogenesis in golden hamster: A flow cytometric evaluation

R. Weissenberg, R. Golan, L. Shochat, Lawrence M. Lewin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


The response of hamster testis to the administration of 450 mg/kg procarbazine (PCB) over a period of 4 weeks was evaluated. Flow cytometry was used to investigate changes in cell populations in testicular single cell suspensions and to correlate these changes with those observed in histological sections. PCB caused significant decrease in testicular and epididymal weight and a drastic reduction in haploid cells and spermatogenic arrest, demonstrating variation among the test animals. The results obtained confirm previous observations concerning detrimental effects of PCB upon spermatogenesis in species such as the rat and mouse, though its effect on hamster testis is milder and does not include the germinal stem cells. The histological evaluation of the testis showed a good correlation with flow cytometric evaluation, emphasizing the usefulness of this method in providing quantitative and rapid results.

Original languageEnglish
Pages (from-to)91-100
Number of pages10
JournalArchives of Andrology
Issue number2
StatePublished - 2002


  • Acridine orange
  • Flow cytometry
  • Hamster
  • Mesocricetus auratus
  • Procarbazine
  • Propidium iodide
  • Spermatogenesis


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