TY - JOUR
T1 - Probiotic strains modulate cytokine production and the immune interplay between human peripheral blood mononucear cells and colon cancer cells
AU - Djaldetti, Meir
AU - Bessler, Hanna
N1 - Publisher Copyright:
© FEMS 2017. All rights reserved.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Human health is tightly connected with a great number of gut microbial cells designated as microbiome or microbiota. We have examined the effect of six microbial strains (MS) included in a commercial probiotic on cytokine production by human peripheral blood mononuclear cells (PBMC) and on their immune dialog with colon carcinoma cells. Non-stimulated and lipopolysaccharide (LPS)-stimulated PBMC were incubated for 24 h with MS. The secretion of tumor necrosis factor alpha (TNFα), IL-1β, IL-6, interferon gamma, IL-10 and IL-1ra and the effect of MS on the immune interplay between PBMC and cells from HT-29 and RKO colon carcinoma lines were evaluated. MS incubated with non-stimulated PBMC induced high expression of all cytokines examined, whereas in those stimulated by LPS, variability in the results was observed. MS added to PBMC co-cultivated with HT-29 or RKO colon cancer cells resulted in downregulation of most cytokines except IL-6 and TNFα, and enhanced production of TNFα and IL-1β. The MS incorporated in the probiotic affected PBMC' cytokine production and altered the cross-talk between immune and colon cancer cells. The results may clarify the way by which probiotics modify the intestinal environment resulting in a decline of colon cancer development.
AB - Human health is tightly connected with a great number of gut microbial cells designated as microbiome or microbiota. We have examined the effect of six microbial strains (MS) included in a commercial probiotic on cytokine production by human peripheral blood mononuclear cells (PBMC) and on their immune dialog with colon carcinoma cells. Non-stimulated and lipopolysaccharide (LPS)-stimulated PBMC were incubated for 24 h with MS. The secretion of tumor necrosis factor alpha (TNFα), IL-1β, IL-6, interferon gamma, IL-10 and IL-1ra and the effect of MS on the immune interplay between PBMC and cells from HT-29 and RKO colon carcinoma lines were evaluated. MS incubated with non-stimulated PBMC induced high expression of all cytokines examined, whereas in those stimulated by LPS, variability in the results was observed. MS added to PBMC co-cultivated with HT-29 or RKO colon cancer cells resulted in downregulation of most cytokines except IL-6 and TNFα, and enhanced production of TNFα and IL-1β. The MS incorporated in the probiotic affected PBMC' cytokine production and altered the cross-talk between immune and colon cancer cells. The results may clarify the way by which probiotics modify the intestinal environment resulting in a decline of colon cancer development.
KW - Colon cancer cells
KW - Cross-talk
KW - Cytokines
KW - Peripheral blood mononuclear cells
KW - Probiotics
UR - http://www.scopus.com/inward/record.url?scp=85016585668&partnerID=8YFLogxK
U2 - 10.1093/femsle/fnx014
DO - 10.1093/femsle/fnx014
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C2 - 28104778
AN - SCOPUS:85016585668
SN - 0378-1097
VL - 364
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 3
M1 - fnx014
ER -