Prevascularization of cardiac patch on the omentum improves its therapeutic outcome

Tal Dvir, Alon Kedem, Emil Ruvinov, Oren Levy, Inbar Freeman, Natalie Landa, Radka Holbova, Micha S. Feinberg, Shani Dror, Yoram Etzion, Jonathan Leor, Smadar Cohen

Research output: Contribution to journalArticlepeer-review

Abstract

The recent progress made in the bioengineering of cardiac patches offers a new therapeutic modality for regenerating the myocardium after myocardial infarction (MI). We present here a strategy for the engineering of a cardiac patch with mature vasculature by heterotopic transplantation onto the omentum. The patch was constructed by seeding neonatal cardiac cells with a mixture of prosurvival and angiogenic factors into an alginate scaffold capable of factor binding and sustained release. After 48 h in culture, the patch was vascularized for 7 days on the omentum, then explanted and transplanted onto infarcted rat hearts, 7 days after MI induction. When evaluated 28 days later, the vascularized cardiac patch showed structural and electrical integration into host myocardium. Moreover, the vascularized patch induced thicker scars, prevented further dilatation of the chamber and ventricular dysfunction. Thus, our study provides evidence that grafting prevascularized cardiac patch into infarct can improve cardiac function after MI.

Original languageEnglish
Pages (from-to)14990-14995
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number35
DOIs
StatePublished - 1 Sep 2009

Keywords

  • Affinity-binding alginate scaffolds
  • Cardiac tissue engineering
  • Myocardial infarction
  • SDF-1
  • Vascularization

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