TY - JOUR
T1 - Preservation of fine structures in yeast by fixation in a dimethyl sulfoxide-acrolein-glutaraldehyde solution
AU - Schwab, D. W.
AU - Janney, A. H.
AU - Scala, J.
AU - Lewin, L. M.
PY - 1970
Y1 - 1970
N2 - The primary fixative containing 2% acrolein, 2% glutaraldehyde in 50% aqueous dimethyl sulfoxide (DMSO) buffered at pH 7.4, was applied for 7 hr in the cold. After a short wash in 0.02 M s-collidine buffer, pH 7.4, containing 0.2 M sucrose and 0.001 M CaCl2, the yeast cells were postfixed in 3% OsO4 at pH 4.0 (veronal acetate buffer). This method preserves many cytoplasmic features such as lipid deposits and ribosomes which are usually destroyed by permanganate fixation. DMSO apparently acts as a permeating agent allowing maximum penetration of the cell wall by the fixative without disrupting cellular fine structure.
AB - The primary fixative containing 2% acrolein, 2% glutaraldehyde in 50% aqueous dimethyl sulfoxide (DMSO) buffered at pH 7.4, was applied for 7 hr in the cold. After a short wash in 0.02 M s-collidine buffer, pH 7.4, containing 0.2 M sucrose and 0.001 M CaCl2, the yeast cells were postfixed in 3% OsO4 at pH 4.0 (veronal acetate buffer). This method preserves many cytoplasmic features such as lipid deposits and ribosomes which are usually destroyed by permanganate fixation. DMSO apparently acts as a permeating agent allowing maximum penetration of the cell wall by the fixative without disrupting cellular fine structure.
UR - http://www.scopus.com/inward/record.url?scp=84907131405&partnerID=8YFLogxK
U2 - 10.3109/10520297009067469
DO - 10.3109/10520297009067469
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 4913302
AN - SCOPUS:84907131405
VL - 45
SP - 143
EP - 147
JO - Biotechnic and Histochemistry
JF - Biotechnic and Histochemistry
SN - 1052-0295
IS - 4
ER -