Presence of O⁶-Methylguanine acceptor protein in the tissues of different classes of vertevrates abd invertebrates

1. 1. We have measured the ability of extracts of tissues from several species of mammals, birds, reptiles, amphibia and fish to demethylate adducts of O⁶-methylguanine in exogenous DNA by transfer of the methyl group to an acceptor protein. Our study also encompassed tissues from a smaller number of invertebrates, from arthropods, molluscs and annelids. 2. 2. The vertebrate tissues used were liver, brain, spleen and kidney. In the case of the invertebrates we sampled liver, neural tissue, gonads, digestive tract and hepatopancreas. 3. 3. There was no consistent change in the amount of acceptor activity per unit of protein or DNA going from cold-blooded to warm-blooded vertebrates. Liver invariably had the highest amount; this finding was not unexpected since metabolic processes in the liver are high, and good cellular protective mechanisms important. 4. 4. Inter-class comparisons within the vertebrates are highly speculative, and hindered by the fact that there is little information on carcinogenesis in animals other than rodents and humans. 5. 5. O⁶-methylguanine aeceptor activity was found in all the invertebrate tissues tested. The amounts were variable, 0.003-0.0051 fmol/μg cellular DNA, but the values fell within the range of those found in the tissues of vertebrates.