Prerequisites for successful human sperm cryobanking: Sperm quality and prefreezing holding time

H. Yavetz*, L. Yogev, Z. Homonnai, G. Paz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Frozen-thawed donor semen was used in artificial inseminations and in vitro fertilization programs. Semen accepted for donation was characterized (mean ± SE) by sperm concentration of 150 ± 18.6 x 106/mL, normal morphology of 57% ± 1.4%, good progressive motility at 1 hour of 57% ± 1.0%, and post-thaw motility of 45% ± 1.0%. Delay of the freezing process for >1 hour after semen delivery caused a deleterious effect to the freezability of sperm. The average monthly fecundability for the 1st 6 months after inseminations was 13.6%. This value decreased dramatically to 2.6% from the 7th month onward. In 74 IVF/embryo transfer (ET) cycles, the fertilization rate was 55.3% ± 3.8%, pregnancy rate (PR) per ET was 39.6%, and the PR per woman was 42.8%.

Original languageEnglish
Pages (from-to)812-816
Number of pages5
JournalFertility and Sterility
Volume55
Issue number4
DOIs
StatePublished - 1991
Externally publishedYes

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