Preparation of protein-bacteriophage conjugates and their use in detection of anti-protein antibodies

Joseph Haimovich, Esther Hurwitz, Noa Novik, Michael Sela

Research output: Contribution to journalArticlepeer-review

Abstract

Protein bacteriophage T4 conjugates can be prepared, making use of bifunctional reagents such as tolylene-2,4-diisocyanate, glutaraldehyde and bis-diazobenzidine. The conjugates surviving the coupling process are inactivated by the specific anti-protein sera. This inactivation can serve as an assay for detection and quantitation of anti-protein antibodies. Using the assay described, as little as 0.2-2 ng of antibody per 1 ml of antiserum could be detected. Insulin bacteriophage conjugate was inactivated to a significant extent by sera of diabetic patients, who had received injections of insulin, diluted 100-10 000-fold.

Original languageEnglish
Pages (from-to)115-124
Number of pages10
JournalBBA - Protein Structure
Volume207
Issue number1
DOIs
StatePublished - 28 Apr 1970
Externally publishedYes

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