TY - JOUR
T1 - Preparation of protein-bacteriophage conjugates and their use in detection of anti-protein antibodies
AU - Haimovich, Joseph
AU - Hurwitz, Esther
AU - Novik, Noa
AU - Sela, Michael
PY - 1970/4/28
Y1 - 1970/4/28
N2 - Protein bacteriophage T4 conjugates can be prepared, making use of bifunctional reagents such as tolylene-2,4-diisocyanate, glutaraldehyde and bis-diazobenzidine. The conjugates surviving the coupling process are inactivated by the specific anti-protein sera. This inactivation can serve as an assay for detection and quantitation of anti-protein antibodies. Using the assay described, as little as 0.2-2 ng of antibody per 1 ml of antiserum could be detected. Insulin bacteriophage conjugate was inactivated to a significant extent by sera of diabetic patients, who had received injections of insulin, diluted 100-10 000-fold.
AB - Protein bacteriophage T4 conjugates can be prepared, making use of bifunctional reagents such as tolylene-2,4-diisocyanate, glutaraldehyde and bis-diazobenzidine. The conjugates surviving the coupling process are inactivated by the specific anti-protein sera. This inactivation can serve as an assay for detection and quantitation of anti-protein antibodies. Using the assay described, as little as 0.2-2 ng of antibody per 1 ml of antiserum could be detected. Insulin bacteriophage conjugate was inactivated to a significant extent by sera of diabetic patients, who had received injections of insulin, diluted 100-10 000-fold.
UR - http://www.scopus.com/inward/record.url?scp=0014965927&partnerID=8YFLogxK
U2 - 10.1016/0005-2795(70)90141-8
DO - 10.1016/0005-2795(70)90141-8
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AN - SCOPUS:0014965927
VL - 207
SP - 115
EP - 124
JO - Biochimica et Biophysica Acta - Proteins and Proteomics
JF - Biochimica et Biophysica Acta - Proteins and Proteomics
SN - 1570-9639
IS - 1
ER -
