TY - JOUR
T1 - Preimplantation aneuploid embryos undergo self-correction in correlation with their developmental potential
AU - Barbash-Hazan, Shiri
AU - Frumkin, Tsvia
AU - Malcov, Mira
AU - Yaron, Yuval
AU - Cohen, Tania
AU - Azem, Foad
AU - Amit, Ami
AU - Ben-Yosef, Dalit
PY - 2009/9
Y1 - 2009/9
N2 - Objective: To investigate the incidence of embryos' self-correction during preimplantation development in terms of mosaicism and in correlation with its developmental stage. Design: Prospective study to compare the chromosome status of embryos on day 3 with that of day 5, in correlation with their developmental stage. Setting: In vitro fertilization unit of a university-affiliated hospital. Patient(s): Eighty-three aneuploid embryos. Intervention(s): Fluorescence in situ hybridization (FISH) reanalysis. Main Outcome Measure(s): Day 3 embryos classified as mosaic or chromosomally abnormal by preimplantation genetic screening (PGS) were reanalyzed on day 5. The results were evaluated in correlation with the embryos' developmental stage. Result(s): Out of 83 day 3 aneuploid embryos, 15 (18.1%) were diagnosed with mosaicism. The FISH reanalysis on day 5 demonstrated that 27 embryos (32.6%) were partly or entirely normal disomic. Of these 83 aneuploid embryos, 8 (9.7%) underwent complete self-correction. The PGS results demonstrated that 26.5% of the embryos were trisomic, of which 41.0% underwent trisomic rescue by day 5. Self-correction was in correlation with the embryo's developmental stage, i.e., 38.1% of aneuploid embryos that developed to the blastocyst stage underwent self-correction compared with only 12.5% of embryos that only cleaved after biopsy. Conclusion(s): Our results demonstrate that self-correction of aneuploid and mosaic embryos occurs probably more significantly during development toward the blastocyst stage than in delayed embryos. In addition, trisomic embryos correct themselves more than other aneuploidies. These findings suggest that PGS results must be interpreted with caution.
AB - Objective: To investigate the incidence of embryos' self-correction during preimplantation development in terms of mosaicism and in correlation with its developmental stage. Design: Prospective study to compare the chromosome status of embryos on day 3 with that of day 5, in correlation with their developmental stage. Setting: In vitro fertilization unit of a university-affiliated hospital. Patient(s): Eighty-three aneuploid embryos. Intervention(s): Fluorescence in situ hybridization (FISH) reanalysis. Main Outcome Measure(s): Day 3 embryos classified as mosaic or chromosomally abnormal by preimplantation genetic screening (PGS) were reanalyzed on day 5. The results were evaluated in correlation with the embryos' developmental stage. Result(s): Out of 83 day 3 aneuploid embryos, 15 (18.1%) were diagnosed with mosaicism. The FISH reanalysis on day 5 demonstrated that 27 embryos (32.6%) were partly or entirely normal disomic. Of these 83 aneuploid embryos, 8 (9.7%) underwent complete self-correction. The PGS results demonstrated that 26.5% of the embryos were trisomic, of which 41.0% underwent trisomic rescue by day 5. Self-correction was in correlation with the embryo's developmental stage, i.e., 38.1% of aneuploid embryos that developed to the blastocyst stage underwent self-correction compared with only 12.5% of embryos that only cleaved after biopsy. Conclusion(s): Our results demonstrate that self-correction of aneuploid and mosaic embryos occurs probably more significantly during development toward the blastocyst stage than in delayed embryos. In addition, trisomic embryos correct themselves more than other aneuploidies. These findings suggest that PGS results must be interpreted with caution.
KW - PGS
KW - Preimplantation embryos
KW - aneuploidy
KW - mosaicism
KW - self-correction
UR - http://www.scopus.com/inward/record.url?scp=69249202535&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2008.07.1761
DO - 10.1016/j.fertnstert.2008.07.1761
M3 - מאמר
AN - SCOPUS:69249202535
VL - 92
SP - 890
EP - 896
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 3
ER -