Pregnancies and live births following ICSI with testicular spermatozoa after repeated implantation failure using ejaculated spermatozoa

Ariel Weissman, Eran Horowitz*, Amir Ravhon, Hana Nahum, Abraham Golan, David Levran

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The use of testicular spermatozoa for IVF/intracytoplasmic sperm injection (ICSI) is currently indicated exclusively for patients with azoospermia, since a favourable outcome is expected even when very few spermatozoa are present in the ejaculate. Here, a series of four couples with long-standing male factor infertility and multiple failed IVF/ICSI cycles are described. In all couples, the use of ejaculated spermatozoa for ICSI resulted in poor embryo quality and repeated implantation failure. Testicular sperm aspiration was performed in subsequent cycles, and testicular spermatozoa were used for ICSI. Embryo implantation and ongoing pregnancies/deliveries were achieved in all four couples. It is postulated that spermatozoa are subjected to post-testicular damage during sperm transport between the seminiferous tubules and epididymis, with the injection of damaged spermatozoa being the cause for repetitive IVF/ICSI failures. In selected patients, the use of testicular spermatozoa for IVF/ICSI should be considered, even when motile spermatozoa can be identified in the ejaculate.

Original languageEnglish
Pages (from-to)605-609
Number of pages5
JournalReproductive BioMedicine Online
Volume17
Issue number5
DOIs
StatePublished - Nov 2008
Externally publishedYes

Keywords

  • DNA fragmentation
  • ICSI
  • IVF
  • Malefactor
  • Repeated implantation failure
  • Testicular spermatozoa

Fingerprint

Dive into the research topics of 'Pregnancies and live births following ICSI with testicular spermatozoa after repeated implantation failure using ejaculated spermatozoa'. Together they form a unique fingerprint.

Cite this