TY - JOUR
T1 - Preferential secretion of a common anti-DNA idiotype (16/6 Id) and anti-polynucleotide antibodies by normal mononuclear cells following stimulation with Klebsiella pneumoniae
AU - El-Roiey, Albert
AU - Gross, Wolfgang L.
AU - Luedemann, Jens
AU - Isenberg, David A.
AU - Shoenfeld, Yehuda
N1 - Funding Information:
Rothschild Fund for the Advancement of Sciences and Technology, and the grant in memory of Yoav Armon, and a grant from the Deutsche Forschungsgemeinschaft (Gr 609/h).
Funding Information:
This study was supported by the Bat-Sheva de
PY - 1986/6
Y1 - 1986/6
N2 - Peripheral blood mononuclear cells derived from 6 normal subjects were incubated with 5 polyclonal activators (pokeweed mitogen, Epstein-Barr virus, group-A Streptococcus, Staphylococcus aureus and Klebsiella pneumoniae. After 7 days of incubation, the supernatants were examined for immunoglobulin (IgG and IgM) production, the level of a common anti-DNA idiotype (16/6 Id) and autoantibody activity against ssDNA, dsDNA, poly(I), poly(dT) and cardiolipin. Significantly increased levels of the 16/6 Id were recorded only with the Klebsiella stimulated MNC. Increased immunoglobulin levels and autoantibody reactivity were noted with all 5 polyclonal activators. The Klebsiella cell membrane preparation induced the highest values. No correlation was found between the 16/6 levels, autoantibody activity and the absolute concentrations of IgG or IgM. This study, together with previous reports, suggests a role for Klebsiella in the etiology of autoimmune diseases. We suggest that Klebsiella can induce anti-DNA-like autoantibodies not only by polyclonal activation, but also by a more specific stimulus.
AB - Peripheral blood mononuclear cells derived from 6 normal subjects were incubated with 5 polyclonal activators (pokeweed mitogen, Epstein-Barr virus, group-A Streptococcus, Staphylococcus aureus and Klebsiella pneumoniae. After 7 days of incubation, the supernatants were examined for immunoglobulin (IgG and IgM) production, the level of a common anti-DNA idiotype (16/6 Id) and autoantibody activity against ssDNA, dsDNA, poly(I), poly(dT) and cardiolipin. Significantly increased levels of the 16/6 Id were recorded only with the Klebsiella stimulated MNC. Increased immunoglobulin levels and autoantibody reactivity were noted with all 5 polyclonal activators. The Klebsiella cell membrane preparation induced the highest values. No correlation was found between the 16/6 levels, autoantibody activity and the absolute concentrations of IgG or IgM. This study, together with previous reports, suggests a role for Klebsiella in the etiology of autoimmune diseases. We suggest that Klebsiella can induce anti-DNA-like autoantibodies not only by polyclonal activation, but also by a more specific stimulus.
KW - Klebsiella pneumoniae polyclonal activation
KW - anti-DNA antibodies
KW - anti-idiotypes
KW - autoantibodies
KW - autoimmunity
UR - http://www.scopus.com/inward/record.url?scp=0022516965&partnerID=8YFLogxK
U2 - 10.1016/0165-2478(86)90036-2
DO - 10.1016/0165-2478(86)90036-2
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0022516965
SN - 0165-2478
VL - 12
SP - 313
EP - 319
JO - Immunology Letters
JF - Immunology Letters
IS - 5-6
ER -
