Prediction of Preeclampsia in Twins Using First Trimester: cffDNA Fraction, PlGF, and MAP

  • Ran Svirsky*
  • , Adi Sharabi-Nov
  • , Ron Maymon
  • , Nadav Kugler
  • , Moran Landau Rabbi
  • , Richard Brown
  • , Heidy Portillo Rodriguez
  • , Linda Peltier
  • , Kypros Nicolaides
  • , Hamutal Meiri
  • *Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: To evaluate cell-free fetal DNA fraction (cffDNAF) as a first-trimester screening marker for preeclampsia necessitating delivery before 37 weeks' gestation in twin pregnancies alone and combined with other bio-markers. Methods: Women with two live fetuses were enrolled in the first trimester, and evaluated for cffDNAF as a first trimester preeclampsia marker alone, and with placental growth factor (PlGF), mean arterial pressure (MAP), and uterine artery pulsatility index (UtA-PI). Results: There were 20 affected women; the cffDNAF was 9.0% (IQR: 8.4%–10.3%) in the affected, compared to 14% (IQR: 11%–16%) in 163 unaffected cases (p < 0.001). The AUROC for cffDNAF was 0.73 (95% CI: 0.61–0.85, p < 0.001), PlGF had an AUROC of 0.71 (0.59–0.83, p = 0.001), MAP had AUROC of 0.61 (0.50–0.72, p = 0.053) whereas UtA-PI had AUROC of 0.54 (0.39–0.69, p > 0.05). Combining all three biomarkers yielded an AUROC of 0.89 (0.78–0.98), with a sensitivity of 81%, specificity of 90%, negative predictive value (NPV) of 97.5%, and positive predictive value (PPV) of 50.7 UtA-PI did not contribute to the AUROC. Conclusion: In twin pregnancies low first trimester cffDNAF effectively screens for preeclampsia necessitating delivery before 37 weeks' gestation, which is augmented with PlGF and MAP.

Original languageEnglish
Pages (from-to)968-978
Number of pages11
JournalPrenatal Diagnosis
Volume45
Issue number8
DOIs
StatePublished - Jul 2025

Funding

FundersFunder number
Israel Ministry of Health Project16874
Canadian Institutes of Health ResearchENP‐168103
Santé294417

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