Precursor of rat liver α2u-globulin: Partial amino acid sequence determination of its signal peptide

Yukio Ikehara, Kimimitsu Oda, Melvin G. Rosenfeld, Shoshana Bar-nun, Gert Kreibich

Research output: Contribution to journalArticlepeer-review

Abstract

The biosynthesis of α2u-globulin, the major protein in rat urine, was studied in a cell-free system programmed with messenger RNA isolated from rat liver. Analysis of the primary translation product by SDS-polyacrylamide gel electrophoresis showed a molecular weight of approximately 22,000 daltons as compared to 20,000 daltons for the mature form. When translation was carried out in the presence of dog pancreas microsomes, α2u-globulin was cotranslationally processed and sequestered in the microsomal lumen. Automated sequencing to mature α2u and the labeled precursor disclosed the presence of 19 additional amino acids at the amino- terminal end of the primary translation product of the urinary α2u The partial amino acid sequence of this extension was determined.

Original languageEnglish
Pages (from-to)1833-1836
Number of pages4
JournalJournal of Biochemistry
Volume90
Issue number6
DOIs
StatePublished - Oct 1981
Externally publishedYes

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