Pre-Disinfection of Poly-Methyl-Methacrylate (PMMA) Reduces Volatile Sulfides Compounds (VSC) Production in Experimental Biofilm In Vitro

Ofir Rosner, Guy Melamed, Shiri Livne, Uziel Jeffet, Eran Dolev, Gil Ben Izhack, Hadas Heller, Nir Sterer

Research output: Contribution to journalArticlepeer-review

Abstract

Temporary dental crowns and bridges are commonly made of poly-methylmethacrylate (PMMA), a porous material attracting the microbial biofilm associated with malodor production. The purpose of the present study was to test pre-disinfection of PMMA on malodor-related parameters in an experimental oral biofilm. PMMA discs were pre-soaked in anti-malodor disinfecting solutions and controls: (i) Saline, (ii) essential oils (EO), (iii) herbal extracts (HE), and (iv) chlorhexidine (CHX). Following, discs were subjected to a salivary incubation assay and monitored for malodor-producing bacteria within the biofilm using confocal microscopy (CLSM), malodor production (organoleptic scale 0–5), volatile sulfide levels (Halimeter), and salivary protein degradation (SDS-PAGE). Results showed that disinfection solutions were significantly effective in reducing malodor-related parameters (CHX > HE > EO > Saline). Taken together, these results suggest that pre-disinfection may help to reduce malodor production in PMMA temporary dental restorations.

Original languageEnglish
Article number1947
JournalApplied Sciences (Switzerland)
Volume12
Issue number4
DOIs
StatePublished - 1 Feb 2022

Keywords

  • Biofilm
  • Malodor
  • Poly-methylmethacrylate

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