Pre-Disinfection of Poly-Methyl-Methacrylate (PMMA) Reduces Volatile Sulfides Compounds (VSC) Production in Experimental Biofilm In Vitro

Ofir Rosner; Guy Melamed; Shiri Livne; Uziel Jeffet; Eran Dolev; Gil Ben Izhack; Hadas Heller; Nir Sterer

doi:10.3390/app12041947

Pre-Disinfection of Poly-Methyl-Methacrylate (PMMA) Reduces Volatile Sulfides Compounds (VSC) Production in Experimental Biofilm In Vitro

Ofir Rosner, Guy Melamed^*, Shiri Livne, Uziel Jeffet, Eran Dolev, Gil Ben Izhack, Hadas Heller, Nir Sterer

^*Corresponding author for this work

School of Dentistry

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Temporary dental crowns and bridges are commonly made of poly-methylmethacrylate (PMMA), a porous material attracting the microbial biofilm associated with malodor production. The purpose of the present study was to test pre-disinfection of PMMA on malodor-related parameters in an experimental oral biofilm. PMMA discs were pre-soaked in anti-malodor disinfecting solutions and controls: (i) Saline, (ii) essential oils (EO), (iii) herbal extracts (HE), and (iv) chlorhexidine (CHX). Following, discs were subjected to a salivary incubation assay and monitored for malodor-producing bacteria within the biofilm using confocal microscopy (CLSM), malodor production (organoleptic scale 0–5), volatile sulfide levels (Halimeter), and salivary protein degradation (SDS-PAGE). Results showed that disinfection solutions were significantly effective in reducing malodor-related parameters (CHX > HE > EO > Saline). Taken together, these results suggest that pre-disinfection may help to reduce malodor production in PMMA temporary dental restorations.

Original language	English
Article number	1947
Journal	Applied Sciences (Switzerland)
Volume	12
Issue number	4
DOIs	https://doi.org/10.3390/app12041947
State	Published - 1 Feb 2022

Keywords

Biofilm
Malodor
Poly-methylmethacrylate

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10.3390/app12041947

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title = "Pre-Disinfection of Poly-Methyl-Methacrylate (PMMA) Reduces Volatile Sulfides Compounds (VSC) Production in Experimental Biofilm In Vitro",

abstract = "Temporary dental crowns and bridges are commonly made of poly-methylmethacrylate (PMMA), a porous material attracting the microbial biofilm associated with malodor production. The purpose of the present study was to test pre-disinfection of PMMA on malodor-related parameters in an experimental oral biofilm. PMMA discs were pre-soaked in anti-malodor disinfecting solutions and controls: (i) Saline, (ii) essential oils (EO), (iii) herbal extracts (HE), and (iv) chlorhexidine (CHX). Following, discs were subjected to a salivary incubation assay and monitored for malodor-producing bacteria within the biofilm using confocal microscopy (CLSM), malodor production (organoleptic scale 0–5), volatile sulfide levels (Halimeter), and salivary protein degradation (SDS-PAGE). Results showed that disinfection solutions were significantly effective in reducing malodor-related parameters (CHX > HE > EO > Saline). Taken together, these results suggest that pre-disinfection may help to reduce malodor production in PMMA temporary dental restorations.",

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author = "Ofir Rosner and Guy Melamed and Shiri Livne and Uziel Jeffet and Eran Dolev and Izhack, {Gil Ben} and Hadas Heller and Nir Sterer",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

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doi = "10.3390/app12041947",

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AU - Rosner, Ofir

AU - Melamed, Guy

AU - Livne, Shiri

AU - Jeffet, Uziel

AU - Dolev, Eran

AU - Izhack, Gil Ben

AU - Heller, Hadas

AU - Sterer, Nir

PY - 2022/2/1

Y1 - 2022/2/1

N2 - Temporary dental crowns and bridges are commonly made of poly-methylmethacrylate (PMMA), a porous material attracting the microbial biofilm associated with malodor production. The purpose of the present study was to test pre-disinfection of PMMA on malodor-related parameters in an experimental oral biofilm. PMMA discs were pre-soaked in anti-malodor disinfecting solutions and controls: (i) Saline, (ii) essential oils (EO), (iii) herbal extracts (HE), and (iv) chlorhexidine (CHX). Following, discs were subjected to a salivary incubation assay and monitored for malodor-producing bacteria within the biofilm using confocal microscopy (CLSM), malodor production (organoleptic scale 0–5), volatile sulfide levels (Halimeter), and salivary protein degradation (SDS-PAGE). Results showed that disinfection solutions were significantly effective in reducing malodor-related parameters (CHX > HE > EO > Saline). Taken together, these results suggest that pre-disinfection may help to reduce malodor production in PMMA temporary dental restorations.

AB - Temporary dental crowns and bridges are commonly made of poly-methylmethacrylate (PMMA), a porous material attracting the microbial biofilm associated with malodor production. The purpose of the present study was to test pre-disinfection of PMMA on malodor-related parameters in an experimental oral biofilm. PMMA discs were pre-soaked in anti-malodor disinfecting solutions and controls: (i) Saline, (ii) essential oils (EO), (iii) herbal extracts (HE), and (iv) chlorhexidine (CHX). Following, discs were subjected to a salivary incubation assay and monitored for malodor-producing bacteria within the biofilm using confocal microscopy (CLSM), malodor production (organoleptic scale 0–5), volatile sulfide levels (Halimeter), and salivary protein degradation (SDS-PAGE). Results showed that disinfection solutions were significantly effective in reducing malodor-related parameters (CHX > HE > EO > Saline). Taken together, these results suggest that pre-disinfection may help to reduce malodor production in PMMA temporary dental restorations.

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KW - Poly-methylmethacrylate

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Pre-Disinfection of Poly-Methyl-Methacrylate (PMMA) Reduces Volatile Sulfides Compounds (VSC) Production in Experimental Biofilm In Vitro

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