Pre-Disinfection of Poly-Methyl-Methacrylate (PMMA) Reduces Volatile Sulfides Compounds (VSC) Production in Experimental Biofilm In Vitro

Ofir Rosner, Guy Melamed*, Shiri Livne, Uziel Jeffet, Eran Dolev, Gil Ben Izhack, Hadas Heller, Nir Sterer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Temporary dental crowns and bridges are commonly made of poly-methylmethacrylate (PMMA), a porous material attracting the microbial biofilm associated with malodor production. The purpose of the present study was to test pre-disinfection of PMMA on malodor-related parameters in an experimental oral biofilm. PMMA discs were pre-soaked in anti-malodor disinfecting solutions and controls: (i) Saline, (ii) essential oils (EO), (iii) herbal extracts (HE), and (iv) chlorhexidine (CHX). Following, discs were subjected to a salivary incubation assay and monitored for malodor-producing bacteria within the biofilm using confocal microscopy (CLSM), malodor production (organoleptic scale 0–5), volatile sulfide levels (Halimeter), and salivary protein degradation (SDS-PAGE). Results showed that disinfection solutions were significantly effective in reducing malodor-related parameters (CHX > HE > EO > Saline). Taken together, these results suggest that pre-disinfection may help to reduce malodor production in PMMA temporary dental restorations.

Original languageEnglish
Article number1947
JournalApplied Sciences (Switzerland)
Volume12
Issue number4
DOIs
StatePublished - 1 Feb 2022

Keywords

  • Biofilm
  • Malodor
  • Poly-methylmethacrylate

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