Potential role of WSB1 isoforms in growth and survival of neuroblastoma cells

Keren Shichrur; Galina Feinberg-Gorenshtein; Drorit Luria; Shifra Ash; Isaac Yaniv; Smadar Avigad

doi:10.1038/pr.2014.2

Potential role of WSB1 isoforms in growth and survival of neuroblastoma cells

Keren Shichrur, Galina Feinberg-Gorenshtein, Drorit Luria, Shifra Ash, Isaac Yaniv, Smadar Avigad^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Background: WD repeat and SOCS box containing protein 1 (WSB1) generates three isoforms that were found to play a role in cancer cell growth and tumor progression. We have studied their expression in neuroblastoma (NB). Methods: The behavior of the expression levels of the WSB1 isoforms was analyzed in NB cell lines, in an in vivo NB xenograft mouse model, and in primary NB tumors using real-time PCR. Effective WSB1 small interfering RNAs were transfected into cultured NB cell lines, and cell viability was analyzed using XTT assay and flow cytometry. Results: A significant predominance of the WSB1 isoform 3 (WSB1₃) expression level was demonstrated in all NB systems examined. Correspondingly, combination of WSB1 3 silencing together with WSB1 isoforms 1+2 silencing in NB cells showed reduced growth, enhanced apoptosis rate, and increased sensitivity to chemotherapeutic agents, specifically related to low expression of WSB1₃. Conclusion: Our results point to a possible differential role of WSB1 isoforms in NB and suggest WSB1³ as a target for therapy in NB.

Original language	English
Pages (from-to)	482-486
Number of pages	5
Journal	Pediatric Research
Volume	75
Issue number	4
DOIs	https://doi.org/10.1038/pr.2014.2
State	Published - Apr 2014

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title = "Potential role of WSB1 isoforms in growth and survival of neuroblastoma cells",

abstract = "Background: WD repeat and SOCS box containing protein 1 (WSB1) generates three isoforms that were found to play a role in cancer cell growth and tumor progression. We have studied their expression in neuroblastoma (NB). Methods: The behavior of the expression levels of the WSB1 isoforms was analyzed in NB cell lines, in an in vivo NB xenograft mouse model, and in primary NB tumors using real-time PCR. Effective WSB1 small interfering RNAs were transfected into cultured NB cell lines, and cell viability was analyzed using XTT assay and flow cytometry. Results: A significant predominance of the WSB1 isoform 3 (WSB13) expression level was demonstrated in all NB systems examined. Correspondingly, combination of WSB1 3 silencing together with WSB1 isoforms 1+2 silencing in NB cells showed reduced growth, enhanced apoptosis rate, and increased sensitivity to chemotherapeutic agents, specifically related to low expression of WSB13. Conclusion: Our results point to a possible differential role of WSB1 isoforms in NB and suggest WSB13 as a target for therapy in NB.",

author = "Keren Shichrur and Galina Feinberg-Gorenshtein and Drorit Luria and Shifra Ash and Isaac Yaniv and Smadar Avigad",

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AU - Shichrur, Keren

AU - Feinberg-Gorenshtein, Galina

AU - Luria, Drorit

AU - Ash, Shifra

AU - Yaniv, Isaac

AU - Avigad, Smadar

PY - 2014/4

Y1 - 2014/4

N2 - Background: WD repeat and SOCS box containing protein 1 (WSB1) generates three isoforms that were found to play a role in cancer cell growth and tumor progression. We have studied their expression in neuroblastoma (NB). Methods: The behavior of the expression levels of the WSB1 isoforms was analyzed in NB cell lines, in an in vivo NB xenograft mouse model, and in primary NB tumors using real-time PCR. Effective WSB1 small interfering RNAs were transfected into cultured NB cell lines, and cell viability was analyzed using XTT assay and flow cytometry. Results: A significant predominance of the WSB1 isoform 3 (WSB13) expression level was demonstrated in all NB systems examined. Correspondingly, combination of WSB1 3 silencing together with WSB1 isoforms 1+2 silencing in NB cells showed reduced growth, enhanced apoptosis rate, and increased sensitivity to chemotherapeutic agents, specifically related to low expression of WSB13. Conclusion: Our results point to a possible differential role of WSB1 isoforms in NB and suggest WSB13 as a target for therapy in NB.

AB - Background: WD repeat and SOCS box containing protein 1 (WSB1) generates three isoforms that were found to play a role in cancer cell growth and tumor progression. We have studied their expression in neuroblastoma (NB). Methods: The behavior of the expression levels of the WSB1 isoforms was analyzed in NB cell lines, in an in vivo NB xenograft mouse model, and in primary NB tumors using real-time PCR. Effective WSB1 small interfering RNAs were transfected into cultured NB cell lines, and cell viability was analyzed using XTT assay and flow cytometry. Results: A significant predominance of the WSB1 isoform 3 (WSB13) expression level was demonstrated in all NB systems examined. Correspondingly, combination of WSB1 3 silencing together with WSB1 isoforms 1+2 silencing in NB cells showed reduced growth, enhanced apoptosis rate, and increased sensitivity to chemotherapeutic agents, specifically related to low expression of WSB13. Conclusion: Our results point to a possible differential role of WSB1 isoforms in NB and suggest WSB13 as a target for therapy in NB.

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Potential role of WSB1 isoforms in growth and survival of neuroblastoma cells

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