TY - JOUR
T1 - Postmortem ultrastructural analysis of a cornea transplanted with descemet membrane endothelial keratoplasty
AU - Livny, Eitan
AU - Parker, Jack S.
AU - Van Der Kaaij, Mariëlle
AU - Haasdijk, Elize D.
AU - Van Der Wees, Jacqueline
AU - Bruinsma, Marieke
AU - Melles, Gerrit R.J.
PY - 2014/8
Y1 - 2014/8
N2 - PURPOSE:: The aim of this study was to describe the ultrastructure of the host-donor interface in the eye of a recently deceased patient, who had undergone Descemet membrane endothelial keratoplasty. METHODS:: The eye was enucleated postmortem, and after standard decontamination, the corneoscleral button was excised, cut into 4 quadrants, and processed for light and transmission electron microscopy evaluation. RESULTS:: Transmission electron microscopy revealed close attachment of the donor's Descemet membrane to the host's stroma and projection of stromal collagen fibers into the interfacial matrix, resembling a normal "virgin" corneal architecture. CONCLUSIONS:: Ultrastructurally, an attached Descemet membrane endothelial keratoplasty graft closely resembles that of an unoperated, healthy eye with no appreciable adventitious or missing structures.
AB - PURPOSE:: The aim of this study was to describe the ultrastructure of the host-donor interface in the eye of a recently deceased patient, who had undergone Descemet membrane endothelial keratoplasty. METHODS:: The eye was enucleated postmortem, and after standard decontamination, the corneoscleral button was excised, cut into 4 quadrants, and processed for light and transmission electron microscopy evaluation. RESULTS:: Transmission electron microscopy revealed close attachment of the donor's Descemet membrane to the host's stroma and projection of stromal collagen fibers into the interfacial matrix, resembling a normal "virgin" corneal architecture. CONCLUSIONS:: Ultrastructurally, an attached Descemet membrane endothelial keratoplasty graft closely resembles that of an unoperated, healthy eye with no appreciable adventitious or missing structures.
KW - DMEK
KW - Descemet membrane
KW - corneal transplantation
KW - electron microscopy
KW - endothelium
KW - ultrastructure
UR - http://www.scopus.com/inward/record.url?scp=84904254099&partnerID=8YFLogxK
U2 - 10.1097/ICO.0000000000000156
DO - 10.1097/ICO.0000000000000156
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C2 - 24977986
AN - SCOPUS:84904254099
SN - 0277-3740
VL - 33
SP - 790
EP - 794
JO - Cornea
JF - Cornea
IS - 8
ER -